This page is produced and maintained by Ray and Peter
Last updated Monday, April 15, 1996 - 12:55:27
10 20 30 40 50 60
AAGCTTGGGCTGCAGAAAAGACCCATGGAAAGGAACAGTCTGTTAGTCTGTCAGCTATTA
^^ ^^ ^^ ^ ^
Hind III Sty I Nsp I Alu I
Alu I Pst I Nco I
Fnu4H I Dsa I
Nla III
70 80 90 100 110 120
TGTCTGGTGGCGCGCGCGGCAGCAACGAGTACTGCTCAGACTACACTGCCCTCCACCGTT
^^^^^^^ ^ ^^ ^ ^ ^^
BssH II Sca I Mnl I Hinc II
Hha I Fnu4H I Rsa I Hind II
Cfo I Bbv I Rru I Dde I Hpa I
HinP I Mse I
Mvn I
Acc II
FnuD II
BstU I
Tha I
Cfo I
HinP I
BssH II
Hha I
BstU I
FnuD II
Acc II
Mvn I
Tha I
HinP I
Hha I
Cfo I
Tha I
FnuD II
Mvn I
Acc II
BstU I
Fnu4H I
130 140 150 160 170 180
AACAGCACCGCAACGGGAGTTACCTCTGACTCTTATCAGAACACAACAACTCAGCTGCCT
^ ^ ^ ^ ^^^
Mae III Dde I
Mnl I Pvu II
Hinf I Alu I
Fnu4H I
190 200 210 220 230 240
GCATCTTCTTCTGCCGCTGCCTTAAGTCTTCCAAATGCGTCAGCGGTGCAAGCCCGCTCC
^ ^ ^ ^^ ^
SfaN I Fnu4H I Ava I
Fnu4H I Nsp III
Bfr I
Afl II
Mse I
250 260 270 280 290 300
CCGAGCTCATTTTCAGACACATACCCTACCGCCACGGCCTTGTGCGGCACACTGGTGGTG
^^ ^ ^^ ^ ^
Bsp 1286 I Dsa I Fnu4H I
Ban II Eae IV Bsr I
Asp HI Cfr I
Sst I Pal I
Sac I Hae III
HgiA I
Nsp II
Alu I
310 320 330 340 350 360
GTGGGCATCGTGCTGTGCCTAAGTCTGGCCTCCACTGTTAGGAGCAAGGAGCTGCCGAGC
^ ^ ^^ ^ ^^
SfaN I Dde I Mnl I Alu I
Eae IV Fnu4H I
Hae I
Cfr I
Pal I
Hae III
370 380 390 400 410 420
GACCATGAGTCGCTGGAGGCATGGGAGCAGGGCTCGGATGTAGAAGCTCCGCCGCTACCG
^ ^ ^ ^ ^ ^ ^
Nla III Nla III Fok I Alu I Msp I
Ple I Fnu4H I
Hinf I Hap II
Hpa II
430 440 450 460 470 480
GAGAAGAGCCCATGTCCGGAACACGTACCCGAGATTCGCGTGGAGATCCCACGTTATGTT
^ ^ ^ ^^ ^^^ ^ ^ ^ ^ ^^ ^ ^
Mbo II Mro I Rsa I Hinf I Mfl I Mse I
Bsp 1286 I Afl III BstU I
Ban II Hap II Nsp III BstY I
Nsp II Acc III Ava I Tha I Nde II
Nla III Bsa I Acc II Mea II
BspM II Mvn I Dpn I
Msp I FnuD II
Hpa II Xho II
Mea II SauIIIA I
Mbo I
490 500 510 520 530 540
TAATAAAAACTGCGGGCACTGGGGACGGTGGTGTTGTATATGTGAATTTGTAAATAATAA
^
Bsr I
550 560 570 580 590 600
ATGAGACCCCATCCTGTAAAAATACAGAGTCCGTGTCAGTCTCTGAAGGACAGTGTATTG
^ ^^ ^
Ple I Nsp I
Hinf I Alw NI
BsmA I
610 620 630 640 650 660
GCATATAGCCAATAAAGAGAGTTGTGGCAAAGAGCCATGTTATGGATTAGTAATGGAAAG
^^
PflM I
Nla III
670 680 690 700 710 720
TATCGTCACCAATAGGGGAGTGGTCAATAATGGTCAATAACCCACACCTATAGGCTAAGC
^ ^ ^^ ^
Mae III Esp I
Xcm I Dde I
Alu I
730 740 750 760 770 780
TATACCATCACCTATAACATGAGGAAGCGGGGGTGTATAGACCCCAAGCCAAAAACAGTA
^
Nla III
790 800 810 820 830 840
TAGCATGCATAAGAAGCCAAGGGGGTGGGCCTATAGACTCTATAGGCGGTACTTACGTCA
^^^ ^ ^^^ ^ ^ ^ ^
Sph I Sty I Pss I Hinf I Rsa I Mae III
Bbu I BstX I Sau96 I Mea II
Nla III Nsp IV
Nsi I Cfr 13I
EcoT22 I Asu I
Hae III
Pal I
850 860 870 880 890 900
CTCTTGGCACGGGGAATCCGCGTTCCAATGCACCGTTCCCGGCCGCGGAGGCTGGATCGG
^ ^ ^^^^^^^ ^^ ^^
Hinf I Dsa V HinGU II
Mvn I ScrF I Alw I
BstU I Bcn I Mbo I
Acc II Nci I SauIIIA I
Tha I Hap II Dpn I
FnuD II Msp I Nde II
Hpa II Pss I
Xma III PpuM I
Ecl X I Asu I
Eae I Nsp IV
Eco52 I Cfr 13I
Eag I Eco47 I
Hae III Sin I
Pal I Ava II
Bgl I Nla IV
Fnu4H I Sau96 I
Dsa I
Ksp I
Sst II
Sac II
BstU I
Acc II
Mvn I
Tha I
FnuD II
910 920 930 940 950 960
TCCCGGTGTCTTCTATGGAGGTCAAAACAGCGTGGATGGCGTCTCCAGGCGATCTGACGG
^^ ^ ^ ^ ^ ^^
ScrF I Fok I Dsa V
Nci I Acy I Mfl I
Dsa V BsmA I SauIIIA I
Bcn I Nci I
Hpa II ScrF I
Msp I Apy I
Hap II Mva I
EcoR II
BstN I
Nde II
Mbo I
Dpn I
970 980 990 1000 1010 1020
TTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGC
^^ ^ ^
Nsp II Mnl I Rsa I
Sac I
Ban II
HgiA I
Bsp 1286 I
Sst I
Asp HI
Alu I
1030 1040 1050 1060 1070 1080
GTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAAC
^ ^
Mae III Nla IV
Ban I
1090 1100 1110 1120 1130 1140
AAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCGCTAT
^^ ^ ^
Aat II Nsp III
Acy I Hinf I
Aha II Ple I
HgiD I
Mea II
1150 1160 1170 1180 1190 1200
CCACGCCCATTGATGTACTGCCAAAACCGCATCACCATGGTAATAGCGATGACTAATACG
^ ^ ^^ ^^
Rsa I SfaN I SnaB I
Dsa I Bsa I
Sty I Eco105 I
Nco I Mea II
Nla III
1210 1220 1230 1240 1250 1260
TAGATGTACTGCCAAGTAGGAAAGTCCCATAAGGTCATGTACTGGGCATAATGCCAGGCG
^ ^ ^ ^ ^^ ^
Rsa I Nla III Bgl I
Rsa I Nci I
Bsr I BstN I
Mva I
EcoR II
Dsa V
Apy I
ScrF I
Nsp IV
Cfr 13I
Asu I
Sau96 I
1270 1280 1290 1300 1310 1320
GGCCATTTACCGTCATTGACGTCAATAGGGGGCGTACTTGGCATATGATACACTTGATGT
^ ^^ ^ ^ ^
Hae III Acy I Rsa I Nde I Rsa I
Pal I HgiD I
Aat II
Aha II
Mea II
1330 1340 1350 1360 1370 1380
ACTGCCAAGTGGGCAGTTTACCGTAAATACTCCACCCATTGACGTCAATGGAAAGTCCCT
^ ^^
Bgl I Aha II
Acy I
Aat II
HgiD I
Mea II
1390 1400 1410 1420 1430 1440
ATTGGCGTTACTATGGGAACATACGTCATTATTGACGTCAATGGGCGGGGGTCGTTGGGC
^ ^ ^^
Mae III Mea II Aha II
Acy I
Aat II
HgiD I
Mea II
1450 1460 1470 1480 1490 1500
GGTCAGCCAGGCGGGCCATTTACCGTAAGTTATGTAACGCGGAACTCCATATATGGGCTA
^^ ^^ ^ ^
Bgl I Pal I Mae III
Mva I Acc II
Apy I FnuD II
BstN I BstU I
Nci I Mvn I
ScrF I Tha I
Dsa V
EcoR II
Nsp IV
Asu I
Cfr 13I
Sau96 I
Hae III
1510 1520 1530 1540 1550 1560
TGAACTAATGACCCCGTAATTGATTACTATTAATAACTAGTCAATAATCAATGTCAACAT
^^ ^^ ^ ^
Ase I /Asn I Hinc II
Mse I Hind II
Spe I Nla III
Mae I
1570 1580 1590 1600 1610 1620
GGCGGTAATGTTGGACATGAGCCAATATAAATGTACATATTATGATATGGATACAACGTA
^ ^ ^
Nla III Rsa I Mea II
1630 1640 1650 1660 1670 1680
TGCAATGGCCAATAGCCAATATTGATTTATGCTATATAACCAATGAATAATATGGCTAAT
^^ ^ ^
Hae I Ssp I Eae IV
Eae I Hae I
Bal I Eae I
Eae IV Bal I
Pal I
Hae III
1690 1700 1710 1720 1730 1740
GGCCAATATTGATTCAATGTATAGATCGATATGCATTGGCCATGTGCCAGCTTGATGTCG
^ ^ ^ ^^^ ^ ^^ ^ ^ ^
Hae III Hinf I Nde II Hae I Alu I Aha II
Pal I SauIIIA I Bal I Bbi II
Ssp I Mbo I Eae IV HgiD I
Dpn I Eae I
Cla I Hae III
Ban III Pal I
Taq I Nla III
Nsi I
EcoT22 I
1750 1760 1770 1780 1790 1800
CCTCTATCGGCGATATAGCCTCATATCGTCTGTCACCTATATCGAAACTGCGATATTTGC
^ ^ ^ ^
Mnl I Mnl I Mae III Taq I
1810 1820 1830 1840 1850 1860
GACACACAGAATCGCCCAAGTCACCAAAGTCGTCTATCGCCATCCCCCGTAAACGATATA
^ ^ ^
Hinf I Mae III
Bbi II
HgiD I
Aha II
1870 1880 1890 1900 1910 1920
AGCCGCTATCGCCAGATATCGCGTATGCCCAAAAATCACTTTTGGAAAAATGGCGATATC
^ ^ ^ ^ ^
Fnu4H I EcoR V EcoR V
FnuD II Nsp I
BstU I
Tha I
Mvn I
Acc II
1930 1940 1950 1960 1970 1980
AGTTACACAGAAACTCACATCGGCGACATTTTCAATATGCCATATTTTCAAATATCGATT
^ ^^
Mae III Cla I
Ban III
Taq I
1990 2000 2010 2020 2030 2040
TTTCCAATATCGCCATCTCTATCGGCGATAAACACCACTATCGCGCGACATGAATTTAGT
^^^ ^
BstU I
Mvn I
Tha I
FnuD II
Acc II
HinP I
Cfo I
Hha I
BstU I
Tha I
Mvn I
Acc II
FnuD II
Nla III
2050 2060 2070 2080 2090 2100
CGGCGACAGAAATCTCAAAACGCGTATTTCGGACAAACACACATTTTATTATTCACTGCA
^^ ^
Afl III Pst I
Mlu I
Tha I
Acc II
Mvn I
BstU I
FnuD II
2110 2120 2130 2140 2150 2160
GGTCGATCGACTCTAGCTAGAGCGGGCCACCTTTGCTATCTTTGCTGAAGATGATGGACC
^^^ ^ ^ ^ ^ ^ ^^ ^ ^^
Taq I Mae I Asu I Mbo II Eco47 I
REM I Alu I Cfr 13I PpuM I
Pvu I Mae I Hae III Pss I
Nde II Sau96 I Sau96 I
Dpn I Nsp IV Asu I
SauIIIA I Pal I Sin I
Mbo I Nsp IV
Taq I Cfr 13I
Hinf I Ava II
Nla IV
2170 2180 2190 2200 2210 2220
CAAACTCGACTTCTGAAGATGTAAAATTTACACCTGACCCATACCAGGTGCCTTTTGTAC
^ ^ ^ ^ ^
Taq I Mbo II BstN I Rsa I
Nci I
Mva I
EcoR II
Apy I
Dsa V
ScrF I
Nla IV
Ban I
2230 2240 2250 2260 2270 2280
AAGCTCTAGCAGACATTGGTGTTCCACAGCCTGCACCAGTGGCTGCCCCGGCACGACGCA
^ ^ ^ ^ ^^ ^
Alu I Alw NI Bcn I Hga I
Mae I Fnu4H I
ScrF I
Nci I
Dsa V
Msp I
Hpa II
Hap II
2290 2300 2310 2320 2330 2340
CACGGAAACCACAACAGCCAGAATCGCTGGAGGAATGCGATTCTGAACTAGAAATAAAGC
^ ^ ^ ^
Hinf I Bsm IwHinf I Mae I
Bsm I
2350 2360 2370 2380 2390 2400
GATACAAGAATCGGGTGGCTTCCAGAAAATGCCGGGCCAAGTTTAAGCAACTGCTGCAGC
^ ^^ ^^ ^ ^^ ^
Hinf I Cfr 10I Mse I Fnu4H I
ScrF I Pst I
Dsa V Fnu4H I
Bcn I Bbv I
Hpa II
Msp I
Hap II
Sau96 I
Cfr 13I
Nsp IV
Asu I
Hae III
Pal I
2410 2420 2430 2440 2450 2460
ACTACCGTGAGGTGGCTGCTGCCAAATCATCTGAAAATGACAGGCTGCGCCTCCTGTTGA
^ ^ ^ ^^ ^
Fnu4H I Fnu4H I
Fnu4H I Hae II
HinP I
Hha I
Cfo I
Mnl I
2470 2480 2490 2500 2510 2520
AGCAGATGTGCCCAAGCCTGGATGTTGCTCTAGGGATCCCCGGGGTCGACCTCGAGCCTA
^ ^ ^ ^ ^^ ^^ ^^ ^^^ ^ ^
Ban II Fok I Mae I Dsa V Mnl I Mae I
Bsp 1286 I Alw I Hinc II Alu I
Nsp II Mva I Bst I Hind II
ScrF I Nla IV Sal I
BstN I Xho II Bbi II
Apy I HinGU II Acc I
Dsa V BamH I Taq I
Nci I BstY I PaeR7 I
EcoR II SauIIIA I Ava I
Mbo I Nsp III
Dpn I Xho I
Nde II Taq I
Xma I
Xcy I
Bcn I
Sma I
Ava I
Nci I
ScrF I
Bcn I
ScrF I
Hpa II
Dsa V
Msp I
Hap II
2530 2540 2550 2560 2570 2580
GCTAGCTAGGCTCGAGGGATCTTTGTGAAGGAACCTTACTTCTGTGGTGTGACATAATTG
^^ ^ ^ ^^ ^^ ^ ^
Nhe I Ava I Nla IV Mae III
Cel II PaeR7 I
Mae I Xho I
Alu I Taq I
Mae I BstY I
Nsp III
Alw I
HinGU II
Xho II
Dpn I
Nde II
Mbo I
SauIIIA I
2590 2600 2610 2620 2630 2640
GACAAACTACCTACAGAGATTTAAAGCTCTAAGGTAAATATAAAATTTTTAAGTGTATAA
^^ ^ ^ ^
Dra I Dde I Mse I
Mse I
Alu I
2650 2660 2670 2680 2690 2700
TGTGTTAAACTACTGATTCTAATTGTTTGTGTATTTTAGATTCCAACCTATGGAACTGAT
^ ^ ^ ^
Mse I Hinf I Hinf I
PflM I
2710 2720 2730 2740 2750 2760
GAATGGGAGCAGTGGTGGAATGCCTTTAATGAGGAAAACCTGTTTTGCTCAGAAGAAATG
^ ^ ^ ^
Bsm Iw Mse I Dde I
Bsm I Mbo II
2770 2780 2790 2800 2810 2820
CCATCTAGTGATGATGAGGCTACTGCTGACTCTCAACATTCTACTCCTCCAAAAAAGAAG
^ ^ ^ ^
Mae I Hinf I Mnl I Mbo II
2830 2840 2850 2860 2870 2880
AGAAAGGTAGAAGACCCCAAGGACTTTCCTTCAGAATTGTCAAGTTTTTTGAGTCATGCT
^ ^ ^ ^
Mbo II Hinf I
Sty I Ple I
Nla III
2890 2900 2910 2920 2930 2940
GTGTTTAGTAATAGAACTCTTGCTTGCTTTGCTATTTACACCACAAAGGAAAAAGCTGCA
^^
Alu I
Fnu4H I
2950 2960 2970 2980 2990 3000
CTGCTATACAAGAAAATTATGGAAAAATATTCTGTAACCTTTATAAGTAGGCATAACAGT
^ ^ ^
Ssp I Mae III Nsp I
3010 3020 3030 3040 3050 3060
TATAATCATAACATACTGTTTTTTCTTACTCCACACAGGCATAGAGTGTCTGCTATTAAT
^^
Ase I /Asn I
Mse I
3070 3080 3090 3100 3110 3120
AACTATGCTCAAAAATTGTGTACCTTTAGCTTTTTAATTTGTAAAGGGGTTAATAAGGAA
^ ^ ^ ^ ^
Rsa I Alu I Mse I Ssp I
Mse I
3130 3140 3150 3160 3170 3180
TATTTGATGTATAGTGCCTTGACTAGAGATCATAATCAGCCATACCACATTTGTAGAGGT
^ ^
Mae I
Dpn I
Mbo I
Mam I
Nde II
SauIIIA I
3190 3200 3210 3220 3230 3240
TTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAACCTGAAACATAAAATGAATGC
^^ ^ ^ ^
Dra I Mnl I Mnl I Bsm Iw
Mse I Bsm I
3250 3260 3270 3280 3290 3300
AATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCAT
^^ ^ ^ ^ ^
Hind II Bbv I Mae III SfaN I
Hpa I Fnu4H I
Hinc II Alu I
Mse I
3310 3320 3330 3340 3350 3360
CACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACT
^
Mae I
3370 3380 3390 3400 3410 3420
CATCAATGTATCTTATCATGTCTGGATCGGAATTCCGTGTATTCTATAGTGTCACCTAAA
^ ^^ ^ ^
Nla III EcoR I Mae III
HinGU II
Alw I
Mbo I
Nde II
SauIIIA I
Dpn I
3430 3440 3450 3460 3470 3480
TCGTATGTGTATGATACATAAGGTTATGTATTAATTGTAGCCGCGTTCTAACGACAATAT
^^ ^ ^
Ase I /Asn I
Mse I Fnu4H I
Acc II
Mvn I
BstU I
FnuD II
Tha I
3490 3500 3510 3520 3530 3540
GTACAAGCCTAATTGTGTAGCATCTGGCTTACTGAAGCAGACCCTATCATCTCTCTCGTA
^ ^
Rsa I SfaN I
3550 3560 3570 3580 3590 3600
AACTGCCGTCAGAGTCGGTTTGGTTGGACGAACCTTCTGAGTTTCTGGTAACGCCGTTCC
^ ^ ^ ^
Aha II Dde I Mae III
HgiD I Hinf I
Bbi II
Ple I
3610 3620 3630 3640 3650 3660
GCACCCCGGAAATGGTCAGCGAACCAATCAGCAGGGTCATCGCTAGCCAGATCCTCTACG
^^ ^^ ^^ ^
Nci I Nhe I SauIIIA I
Dsa V Cel II Mnl I
ScrF I Mae I
Bcn I Mfl I
Hap II BstY I
Msp I Xho II
Hpa II Mbo I
Nde II
Dpn I
3670 3680 3690 3700 3710 3720
CCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCG
^ ^ ^ ^^^^ ^ ^ ^^ ^^ ^
Msp I Eae I Msp I Nar I Bbi II
Hpa II Hae III Hpa II Ehe I
Hap II Pal I Hap II Ban I
Hga I Nae I Bbe I Acy I
SfaN I Hap II Ban I Bbe I
Hpa II Nla IV Nla IV
Msp I Nar I Hha I
SfaN I Cfo I Cfo I
Ehe I HinP I
Acy I
HinP I
Hha I
3730 3740 3750 3760 3770 3780
CCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCG
^ ^ ^^ ^^
Mbo II BspH I
Nde II Nla III
SauIIIA I Eco 47 III
Dpn I Hae II
Mbo I HinP I
Hha I
Cfo I
3790 3800 3810 3820 3830 3840
GCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATG
^^ ^^ ^^^^ ^^ ^^
Pss I Eae I Nla IV Sph I
Eco0 109 I Acy I Bbu I
Cfr I Pal I Nar I Nla III
Dra II Hae III Ban I
Hae III Dsa V Ehe I
Sau96 I Hpa II Bbe I
Pal I Cfr 10I Hha I
Asu I Msp I HinP I
Nsp IV ScrF I Cfo I
Cfr 13I Hap II
Nla IV Bcn I
Nsp III
Dsa I
3850 3860 3870 3880 3890 3900
CACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAA
^ ^ ^ ^^ ^ ^ ^ ^
Fnu4H I Eae IV Bsr I EcoN I
Fnu4H I Cfr I Fnu4H I
Bsp 1286 I
Nsp II Pal I
HgiA I Mnl I
Ban II
Asp HI Hae III
3910 3920 3930 3940 3950 3960
TGCAGGAGTCGCATAAGGGAGAGCGTCGATATGGTGCACTCTCAGTACAATCTGCTCTGA
^ ^ ^ ^ ^
Hinf I Taq I Nsp II Rsa I
Ple I Asp HI
Bsp 1286 I
Sno I
HgiA I
Apa LI
Dde I
3970 3980 3990 4000 4010 4020
TGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGC
^ ^ ^ ^^ ^
Fnu4H I Hga I Drd I
Mse I Mvn I
Acc II
Tha I
Hae II
FnuD II
BstU I
Ban II
Nsp II
HinP I
Cfo I
Hha I
Bsp 1286 I
4030 4040 4050 4060 4070 4080
TTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTG
^^ ^ ^ ^ ^ ^ ^^ ^
ScrF I Alu I BsmA I Alu I
Dsa V Mae III Dsa V Nla III
Nci I Hap II
Bcn I Msp I
Hpa II Bcn I
Hap II Hpa II
Msp I ScrF I
SfaN I Fnu4H I
4090 4100 4110 4120 4130 4140
TCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCT
^^^ ^^^ ^^
FnuD II Eco0 109 I
Acc II Dra II
BstU I Hae III
Mvn I Pss I
Tha I Asu I
HinP I Cfr 13I
Cfo I Nsp IV
Hha I Sau96 I
FnuD II Pal I
BstU I Mnl I
Mvn I Nsp III
Tha I
Acc II
4150 4160 4170 4180 4190 4200
ATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCG
^ ^^ ^ ^^
Mse I Dde I
BspH I HgiD I
Nla III Acy I
Aat II
Aha II
Mea II
4210 4220 4230 4240 4250 4260
GGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCC
^^ ^
Hha I
HinP I
Cfo I
BstU I
FnuD II
Mvn I
Acc II
Tha I
Nla IV
4270 4280 4290 4300 4310 4320
GCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAG
^^ ^ ^
BspH I Ssp I Mbo II
Nla III
4330 4340 4350 4360 4370 4380
TATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTT
^ ^
Aha II Fnu4H I
HgiD I
Bbi II
4390 4400 4410 4420 4430 4440
TGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGT
^ ^ ^ ^ ^
Hph I Mbo II Asp HI
Nde II Nsp II
Dpn I Sno I
SauIIIA I
Mbo I Apa LI
Nsp I
HgiA I
Bsp 1286 I
4450 4460 4470 4480 4490 4500
GGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGA
^ ^ ^ ^^ ^^ ^ ^
Mae III Bsr I Xho II Mbo II
Taq I Dpn I Mfl I Xmn I
BstY I BstY I Asp 700
HinGU II Mbo I
Xho II Nde II
Alw I Dpn I
Nde II SauIIIA I
SauIIIA I
Mbo I
4510 4520 4530 4540 4550 4560
ACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTAT
^ ^ ^^ ^^
Mea II HgiA I Cfo I
Asp HI HinP I
Bsp 1286 I Hha I
Nsp II Mvn I
Dra I BstU I
Mse I Tha I
FnuD II
Acc II
4570 4580 4590 4600 4610 4620
TGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGA
^ ^^ ^ ^ ^ ^
HgiD I Aha II Dde I Rru I
Acy I HgiD I Sca I
Aha II Bbi II
Hga I Fnu4H I
Cfr 10I
Hap II
Dsa V
Msp I
Bcn I
Hpa II
ScrF I
4630 4640 4650 4660 4670 4680
GTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAG
^ ^ ^ ^ ^ ^
Rsa I Mae III SfaN I Fok I
Nsp I Nla III
4690 4700 4710 4720 4730 4740
TGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGG
^ ^ ^^^ ^^ ^
Fnu4H I Nla III Fnu4H I Pvu I Cfr 13I
Eae I SauIIIA I
Eae IV Mbo I
Hae III Nde II
Pal I Dpn I
Asu I
Nsp IV
Sau96 I
Ava II
Eco47 I
Sin I
4750 4760 4770 4780 4790 4800
ACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCG
^ ^ ^^ ^ ^ ^
Alu I Nla III Nla III SauIIIA I
Alw I Dpn I
HinGU II Nde II
SauIIIA I Mbo I
Mbo I
Nde II
Dpn I
Mae III
4810 4820 4830 4840 4850 4860
TTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGT
^ ^ ^ ^
Nla IV Mae III
Hap II
Hpa II
Msp I
Alu I
4870 4880 4890 4900 4910 4920
AGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCG
^ ^^ ^ ^ ^ ^ ^^
Mea II Mse I Mae I Hpa II
Fdi II Bsr I Alu I
Aos I Nci I
Mst I Bcn I
Fsp I Dsa V
Avi II ScrF I
Hha I Msp I
Cfo I Hap II
HinP I
4930 4940 4950 4960 4970 4980
GCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGC
^^ ^ ^ ^ ^^ ^^^
Ase I /Asn I Eco47 I Hae II
Mse I Fok I Sin I Ban II
Bsr I Cfr 13I Bsp 1286 I
Asu I HinP I
Ava II Nsp II
Nsp IV Cfo I
Sau96 I Hha I
Cfr I
Eae IV
Pss I
Hae III
Sau96 I
Pal I
Cfr 13I
Nsp IV
Asu I
Bgl I
4990 5000 5010 5020 5030 5040
CCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGG
^ ^ ^^ ^ ^ ^ ^
Hap II Nla IV BsmA I
Msp I Cfr 10I Nsp III
Hpa II Hap II Mvn I
Msp I BstU I
Hpa II Tha I
Hph I Acc II
FnuD II
5050 5060 5070 5080 5090 5100
TATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGAC
^ ^ ^^^ ^
Fnu4H I Hae III Mnl I
Bbv I Nla IV
Bsr I
Sau96 I
Nsp IV
Asu I
Cfr 13I
Pal I
5110 5120 5130 5140 5150 5160
GGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACT
^ ^ ^ ^ ^ ^
Hinf I Fok I Nde II Ban I
Ple I Mbo I Nla IV
Dpn I Mnl I
SauIIIA I
Dde I
5170 5180 5190 5200 5210 5220
GATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAA
^ ^ ^^
Mse I Mae III Dra I
Mse I
5230 5240 5250 5260 5270 5280
ACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAA
^ ^^ ^^ ^ ^ ^ ^^ ^^
Mse I HinGU II Mbo II BspH I
Dra I Dpn I Mfl I Nla III
Mse I Mae I Xho II
Xho II BstY I
Alw I Dpn I
BstY I
Mbo I Mbo I
Nde II Nde II
SauIIIA I SauIIIA I
Hph I
5290 5300 5310 5320 5330 5340
AATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGG
^ ^ ^ ^ ^^
Mse I Dde I Mbo I SauIIIA I
Mea II SauIIIA I
Dpn I Nde II
Nde II
Alw I
BstY I
Xho II
HinGU II
Dpn I
Mbo I
5350 5360 5370 5380 5390 5400
ATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACC
^^ ^^ ^
BstY I HinP I Fnu4H I
Mfl I Hha I
Xho II Cfo I
Dpn I Tha I
Nde II BstU I
Mbo I Mvn I
SauIIIA I Acc II
FnuD II
5410 5420 5430 5440 5450 5460
GCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAAC
^ ^^ ^ ^ ^
Msp I Alu I Mae III
Hap II Bsr I
Hpa II
Alw I
HinGU II
Mbo I
Dpn I
Nde II
SauIIIA I
5470 5480 5490 5500 5510 5520
TGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCA
^ ^ ^^
Cfo I Mae I Hae I
Hha I Hae III
HinP I Pal I
5530 5540 5550 5560 5570 5580
CCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGT
^ ^ ^
Mnl I Mae III
Alw NI
5590 5600 5610 5620 5630 5640
GGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACC
^ ^ ^^ ^ ^ ^
Fnu4H I Cfr 10I Mae III
Fnu4H I Bcn I Hinf I Msp I
Msp I Hpa II
Hap II Hap II
ScrF I
Hpa II
Dsa V
5650 5660 5670 5680 5690 5700
GGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCG
^ ^ ^ ^
Cfo I Nsp II Alu I
Hha I Sno I
HinP I Bsp 1286 I
Fnu4H I Apa LI
Bbv I HgiA I
Asp HI
5710 5720 5730 5740 5750 5760
AACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCC
^ ^ ^^
Dde I Alu I Hae II
Cfo I
Hha I
HinP I
5770 5780 5790 5800 5810 5820
CGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCAC
^ ^ ^
Msp I Bsp 1286 I
Hap II Hha I
Hpa II HinP I
Fnu4H I Cfo I
Nsp II
5830 5840 5850 5860 5870 5880
GAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCT
^ ^ ^ ^
Alu I Dsa V Mnl I
Apy I EcoR II
Nci I Mva I
ScrF I BstN I
EcoR II ScrF I
Dsa V Nci I
Mva I Apy I
BstN I
5890 5900 5910 5920 5930 5940
CTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGC
^ ^ ^
Drd I Taq I Nla IV
5950 5960 5970 5980 5990 6000
CAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTT
^^^^ ^ ^ ^^ ^^ ^^
FnuD II Nla IV Eae IV Afl III
Mvn I Nci I Hae I Nla III
Tha I ScrF I Cfr I
Acc II Apy I Pal I
BstU I Mva I Hae III
Fnu4H I BstN I
Cfr I EcoR II
Eae IV Dsa V
Hae III Eae IV
Pal I Cfr I
Hae I
Pal I
Hae III
6010 6020 6030 6040 6050 6060
TCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATAC
^ ^
Hinf I Alu I
6070 6080 6090 6100 6110 6120
CGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCG
^ ^ ^ ^ ^ ^ ^^
Fnu4H I HinP I Mbo II
Bbv I Cfo I Hae II
Fnu4H I Hha I Cfo I
Fnu4H I Hha I
Bbv I Ban II
Ple I Nsp II
Hinf I HinP I
Bsp 1286 I
6130 6140 6150 6160 6170 6180
CCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCTTAT
^ ^ ^^^ ^^ ^ ^^ ^^^ ^
Mnl I Tha I Hae III Mse I Taq I
Nsp III Pal I Ase I /Asn I
BstU I Fnu4H I
Mvn I Hinf I Bbv I
FnuD II Pvu II
Acc II Alu I
Hha I
HinP I
Cfo I
Tha I
BstU I
Acc II
Mvn I
FnuD II
Eae I
6190 6200 6210
CGAAATTAATACGACTCACTATAGGGAGACCGG
^^ ^ ^
Ase I /Asn I Hap II
Mse I Msp I
Hinf I Hpa II