1. Run gel using TEA Buffer.
2. Slice band & weigh.
3. Add 3 volumes of NaI solution (3ml/1g of gel).
4. Heat @ 55C until agarose is dissolved (app. 10 min).
5. Add 2-3 µl of glass, mix well, leave 5 min @ RT.
6. Pellet 3' @ 4K rpm, remove supernatant.
7. Resuspend in 200 µl NaI solution.
8. Pellet 3' @ 4K rpm.
9. Wash 3 times in 200 µl EtOH wash. (Pellet 3min @ 4 K each time
except last when you need to spin 3min @ 14 K rpm).
10. Wipe sides of the tube and let dry 5-30 min.
11. Resuspend in 30 µl of H₂O.
12. Elute @ 55C 5 min.
13. Pellet 1' @ 14K RPM.
14. Remove 27 µl of supernatant (ready to use).

***Alternatively if two fragments are used for ligation (besides vector) it can be resuspended in 15 µl and 13 µl removed. In that case use 6.5 µl for ligation so that total volume is the same.