This page is produced and maintained by Ray and Peter
Last updated Monday, April 15, 1996 - 12:55:54
10 20 30 40 50 60
AAGCTTGGGCTGCAGAAAAGACCCATGGAAAGGAACAGTCTGTTAGTCTGTCAGCTATTA
^^ ^^ ^^ ^ ^
Hind III Dsa I Nsp I Alu I
Alu I Pst I Nco I
Fnu4H I Sty I
Nla III
70 80 90 100 110 120
TGTCTGGTGGCGCGCGCGGCAGCAACGAGTACTGCTCAGACTACACTGCCCTCCACCGTT
^^^^^^^ ^ ^^ ^ ^ ^^
Cfo I Fnu4H I Rsa I Mnl I Hinc II
HinP I Bbv I Rru I Dde I Hind II
BssH II Sca I Hpa I
Hha I Mse I
FnuD II
BstU I
Mvn I
Tha I
Acc II
HinP I
Cfo I
BssH II
Hha I
BstU I
Tha I
FnuD II
Acc II
Mvn I
Cfo I
Hha I
HinP I
Acc II
Tha I
BstU I
Mvn I
FnuD II
Fnu4H I
130 140 150 160 170 180
AACAGCACCGCAACGGGAGTTACCTCTGACTCTTATCAGAACACAACAACTCAGCTGCCT
^ ^ ^ ^ ^^^
Mae III Dde I
Mnl I Pvu II
Hinf I Alu I
Fnu4H I
190 200 210 220 230 240
GCATCTTCTTCTGCCGCTGCCTTAAGTCTTCCAAATGCGTCAGCGGTGCAAGCCCGCTCC
^ ^ ^ ^^ ^
SfaN I Fnu4H I Nsp III
Fnu4H I Ava I
Afl II
Bfr I
Mse I
250 260 270 280 290 300
CCGAGCTCATTTTCAGACACATACCCTACCGCCACGGCCTTGTGCGGCACACTGGTGGTG
^^ ^ ^^ ^ ^
Asp HI Dsa I Fnu4H I
Ban II Cfr I Bsr I
Nsp II Eae IV
HgiA I Pal I
Bsp 1286 I Hae III
Sst I
Sac I
Alu I
310 320 330 340 350 360
GTGGGCATCGTGCTGTGCCTAAGTCTGGCCTCCACTGTTAGGAGCAAGGAGCTGCCGAGC
^ ^ ^^ ^ ^^
SfaN I Dde I Mnl I Alu I
Eae IV Fnu4H I
Hae I
Cfr I
Hae III
Pal I
370 380 390 400 410 420
GACCATGAGTCGCTGGAGGCATGGGAGCAGGGCTCGGATGTAGAAGCTCCGCCGCTACCG
^ ^ ^ ^ ^ ^ ^
Nla III Nla III Fok I Alu I Hap II
Hinf I Fnu4H I
Ple I Msp I
Hpa II
430 440 450 460 470 480
GAGAAGAGCCCATGTCCGGAACACGTACCCGAGATTCGCGTGGAGATCCCACGTTATGTT
^ ^ ^ ^^ ^^^ ^ ^ ^ ^ ^^ ^ ^
Mbo II BspM II Rsa I Hinf I Xho II Mse I
Nsp II Mro I Ava I Tha I Nde II
Bsp 1286 I Afl III BstU I
Ban II Hpa II Nsp III BstY I
Nla III Bsa I Mvn I SauIIIA I
Acc III FnuD II Mea II
Msp I Acc II
Hap II Mfl I
Mea II Dpn I
Mbo I
490 500 510 520 530 540
TAATAAAAACTGCGGGCACTGGGGACGGTGGTGTTGTATATGTGAATTTGTAAATAATAA
^
Bsr I
550 560 570 580 590 600
ATGAGACCCCATCCTGTAAAAATACAGAGTCCGTGTCAGTCTCTGAAGGACAGTGTATTG
^ ^^ ^
Ple I Nsp I
Hinf I Alw NI
BsmA I
610 620 630 640 650 660
GCATATAGCCAATAAAGAGAGTTGTGGCAAAGAGCCATGTTATGGATTAGTAATGGAAAG
^^
PflM I
Nla III
670 680 690 700 710 720
TATCGTCACCAATAGGGGAGTGGTCAATAATGGTCAATAACCCACACCTATAGGCTAAGC
^ ^ ^^ ^
Mae III Esp I
Xcm I Dde I
Alu I
730 740 750 760 770 780
TATACCATCACCTATAACATGAGGAAGCGGGGGTGTATAGACCCCAAGCCAAAAACAGTA
^
Nla III
790 800 810 820 830 840
TAGCATGCATAAGAAGCCAAGGGGGTGGGCCTATAGACTCTATAGGCGGTACTTACGTCA
^^^ ^ ^^^ ^ ^ ^ ^
Bbu I BstX I Sau96 I Rsa I Mae III
Sph I Sty I Pss I Hinf I Mea II
Nla III Cfr 13I
EcoT22 I Asu I
Nsi I Nsp IV
Pal I
Hae III
850 860 870 880 890 900
CTCTTGGCACGGGGAATCCGCGTTCCAATGCACCGTTCCCGGCCGCGGAGGCTGGATCGG
^ ^ ^^^^^^^ ^^ ^^
Hinf I Bcn I HinGU II
FnuD II Nci I Alw I
Acc II ScrF I Mbo I
Tha I Dsa V Nde II
BstU I Msp I Dpn I
Mvn I Hpa II SauIIIA I
Hap II Pss I
Eae I PpuM I
Ecl X I Eco47 I
Eag I Ava II
Xma III Nla IV
Eco52 I Asu I
Pal I Nsp IV
Hae III Sau96 I
Fnu4H I Cfr 13I
Bgl I Sin I
Ksp I
Sst II
Dsa I
Sac II
BstU I
Mvn I
Acc II
FnuD II
Tha I
910 920 930 940 950 960
TCCCGGTGTCTTCTATGGAGGTCAAAACAGCGTGGATGGCGTCTCCAGGCGATCTGACGG
^^ ^ ^ ^ ^ ^^
Nci I Fok I Apy I
ScrF I Acy I Mfl I
Dsa V BsmA I Dpn I
Bcn I Mva I
Msp I BstN I
Hpa II EcoR II
Hap II ScrF I
Dsa V
Nci I
SauIIIA I
Mbo I
Nde II
970 980 990 1000 1010 1020
TTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGC
^^ ^ ^
Nsp II Mnl I Rsa I
Ban II
Sst I
Sac I
Asp HI
HgiA I
Bsp 1286 I
Alu I
1030 1040 1050 1060 1070 1080
GTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAAC
^ ^
Mae III Ban I
Nla IV
1090 1100 1110 1120 1130 1140
AAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCGCTAT
^^ ^ ^
Aha II Nsp III
Aat II Hinf I
HgiD I Ple I
Acy I
Mea II
1150 1160 1170 1180 1190 1200
CCACGCCCATTGATGTACTGCCAAAACCGCATCACCATGGTAATAGCGATGACTAATACG
^ ^ ^^ ^^
Rsa I SfaN I Bsa I
Dsa I Eco105 I
Sty I SnaB I
Nco I Mea II
Nla III
1210 1220 1230 1240 1250 1260
TAGATGTACTGCCAAGTAGGAAAGTCCCATAAGGTCATGTACTGGGCATAATGCCAGGCG
^ ^ ^ ^ ^^ ^
Rsa I Nla III Bgl I
Rsa I Nci I
Bsr I ScrF I
BstN I
Apy I
Dsa V
EcoR II
Mva I
Asu I
Sau96 I
Nsp IV
Cfr 13I
1270 1280 1290 1300 1310 1320
GGCCATTTACCGTCATTGACGTCAATAGGGGGCGTACTTGGCATATGATACACTTGATGT
^ ^^ ^ ^ ^
Hae III Aat II Rsa I Nde I Rsa I
Pal I Acy I
Aha II
HgiD I
Mea II
1330 1340 1350 1360 1370 1380
ACTGCCAAGTGGGCAGTTTACCGTAAATACTCCACCCATTGACGTCAATGGAAAGTCCCT
^ ^^
Bgl I Aha II
Acy I
Aat II
HgiD I
Mea II
1390 1400 1410 1420 1430 1440
ATTGGCGTTACTATGGGAACATACGTCATTATTGACGTCAATGGGCGGGGGTCGTTGGGC
^ ^ ^^
Mae III Mea II Aha II
HgiD I
Aat II
Acy I
Mea II
1450 1460 1470 1480 1490 1500
GGTCAGCCAGGCGGGCCATTTACCGTAAGTTATGTAACGCGGAACTCCATATATGGGCTA
^^ ^^ ^ ^
Bgl I Hae III Mae III
Mva I Acc II
Apy I FnuD II
Nci I Tha I
BstN I Mvn I
ScrF I BstU I
Dsa V
EcoR II
Sau96 I
Nsp IV
Asu I
Cfr 13I
Pal I
1510 1520 1530 1540 1550 1560
TGAACTAATGACCCCGTAATTGATTACTATTAATAACTAGTCAATAATCAATGTCAACAT
^^ ^^ ^ ^
Ase I /Asn I Hind II
Mse I Hinc II
Spe I Nla III
Mae I
1570 1580 1590 1600 1610 1620
GGCGGTAATGTTGGACATGAGCCAATATAAATGTACATATTATGATATGGATACAACGTA
^ ^ ^
Nla III Rsa I Mea II
1630 1640 1650 1660 1670 1680
TGCAATGGCCAATAGCCAATATTGATTTATGCTATATAACCAATGAATAATATGGCTAAT
^^ ^ ^
Eae IV Ssp I Hae I
Hae I Eae IV
Eae I Eae I
Bal I Bal I
Pal I
Hae III
1690 1700 1710 1720 1730 1740
GGCCAATATTGATTCAATGTATAGATCGATATGCATTGGCCATGTGCCAGCTTGATGTCG
^ ^ ^ ^^^ ^ ^^ ^ ^ ^
Pal I Hinf I Mbo I Bal I Alu I Aha II
Hae III Nde II Eae IV HgiD I
Ssp I SauIIIA I Eae I Bbi II
Dpn I Hae I
Cla I Pal I
Ban III Hae III
Taq I Nla III
Nsi I
EcoT22 I
1750 1760 1770 1780 1790 1800
CCTCTATCGGCGATATAGCCTCATATCGTCTGTCACCTATATCGAAACTGCGATATTTGC
^ ^ ^ ^
Mnl I Mnl I Mae III Taq I
1810 1820 1830 1840 1850 1860
GACACACAGAATCGCCCAAGTCACCAAAGTCGTCTATCGCCATCCCCCGTAAACGATATA
^ ^ ^
Hinf I Mae III
Bbi II
Aha II
HgiD I
1870 1880 1890 1900 1910 1920
AGCCGCTATCGCCAGATATCGCGTATGCCCAAAAATCACTTTTGGAAAAATGGCGATATC
^ ^ ^ ^ ^
Fnu4H I EcoR V EcoR V
BstU I Nsp I
Acc II
Mvn I
FnuD II
Tha I
1930 1940 1950 1960 1970 1980
AGTTACACAGAAACTCACATCGGCGACATTTTCAATATGCCATATTTTCAAATATCGATT
^ ^^
Mae III Cla I
Ban III
Taq I
1990 2000 2010 2020 2030 2040
TTTCCAATATCGCCATCTCTATCGGCGATAAACACCACTATCGCGCGACATGAATTTAGT
^^^ ^
Mvn I
BstU I
FnuD II
Tha I
Acc II
HinP I
Hha I
Cfo I
Acc II
Tha I
BstU I
Mvn I
FnuD II
Nla III
2050 2060 2070 2080 2090 2100
CGGCGACAGAAATCTCAAAACGCGTATTTCGGACAAACACACATTTTATTATTCACTGCA
^^ ^
Mlu I Pst I
Afl III
BstU I
Mvn I
FnuD II
Tha I
Acc II
2110 2120 2130 2140 2150 2160
GGTCGATCGACTCTAGCTAGAGCAGGGCCACCTTTGCTATCTTTGCTGAAGATGATGGAC
^^^ ^ ^ ^ ^ ^ ^^ ^ ^^
REM I Mae I Sau96 I Mbo II Sau96 I
Taq I Alu I Asu I Pss I
Pvu I Mae I Nsp IV PpuM I
Dpn I Cfr 13I Sin I
Mbo I Pal I Eco47 I
SauIIIA I Hae III Asu I
Nde II Cfr 13I
Taq I Nla IV
Hinf I Nsp IV
Ava II
2170 2180 2190 2200 2210 2220
CCAAACTCGACTTCTGAAGATGTAAAATTTACACCTGACCCATACCAGGTGCCTTTTGTA
^ ^ ^ ^ ^
Taq I Mbo II Mva I Rsa I
Nci I
BstN I
EcoR II
Apy I
ScrF I
Dsa V
Nla IV
Ban I
2230 2240 2250 2260 2270 2280
CAAGCTCTAGCAGACATTGGTGTTCCACAGCCTGCACCAGTGGCTGCCCCGGCACGACGC
^ ^ ^ ^ ^^ ^
Alu I Alw NI Nci I Hga I
Mae I Fnu4H I
Bcn I
ScrF I
Dsa V
Msp I
Hap II
Hpa II
2290 2300 2310 2320 2330 2340
ACACGGAAACCACAACAGCCAGAATCGCTGGAGGAATGCGATTCTGAACTAGAAATAAAG
^ ^ ^ ^
Hinf I Bsm IwHinf I Mae I
Bsm I
2350 2360 2370 2380 2390 2400
CGATACAAGAATCGGGTGGCTTCCAGAAAATGCCGGGCCAAGTTTAAGCAACTGCTGCAG
^ ^^ ^^ ^ ^^ ^
Hinf I Cfr 10I Mse I Fnu4H I
Msp I Pst I
Bcn I Bbv I
ScrF I Fnu4H I
Dsa V
Hpa II
Hap II
Sau96 I
Asu I
Nsp IV
Cfr 13I
Hae III
Pal I
2410 2420 2430 2440 2450 2460
CACTACCGTGAGGTGGCTGCTGCCAAATCATCTGAAAATGACAGGCTGCGCCTCCTGTTG
^ ^ ^ ^^ ^
Fnu4H I Fnu4H I
Fnu4H I Hae II
Hha I
HinP I
Cfo I
Mnl I
2470 2480 2490 2500 2510 2520
AAGCAGATGTGCCCAAGCCTGGATGTTTGCTCTAGAGGATCCCCGGGGTCGACCTCGAGC
^ ^ ^ ^^ ^^ ^^ ^^ ^^^
Nsp II Mva I Xba I Xma I Mnl I
Bsp 1286 I Mae I Dsa V Nsp III
Ban II Fok I Bst I Hind II
ScrF I Alw I Hinc II
BstN I Xho II Bbi II
Apy I BstY I PaeR7 I
Dsa V HinGU II Sal I
EcoR II Nla IV Acc I
Nci I BamH I Taq I
SauIIIA I Xho I
Mbo I Ava I
Dpn I Taq I
Nde II
Bcn I
ScrF I
Sma I
Ava I
Xcy I
Nci I
Hpa II
Dsa V
Msp I
Bcn I
Hap II
ScrF I
2530 2540 2550 2560 2570 2580
CTAGCTAGCTAGGCTCGAGGGATCTTTGTGAAGGAACCTTACTTCTGTGGTGTGACATAA
^ ^^^ ^ ^ ^^ ^^ ^ ^
Mae I Mae I Alw I Nla IV Mae III
Alu I Ava I
Nhe I PaeR7 I
Cel II Nsp III
Mae I Xho I
Alu I Taq I
BstY I
Xho II
HinGU II
Dpn I
Nde II
Mbo I
SauIIIA I
2590 2600 2610 2620 2630 2640
TTGGACAAACTACCTACAGAGATTTAAAGCTCTAAGGTAAATATAAAATTTTTAAGTGTA
^^ ^ ^ ^
Dra I Dde I Mse I
Mse I
Alu I
2650 2660 2670 2680 2690 2700
TAATGTGTTAAACTACTGATTCTAATTGTTTGTGTATTTTAGATTCCAACCTATGGAACT
^ ^ ^ ^
Mse I Hinf I Hinf I
PflM I
2710 2720 2730 2740 2750 2760
GATGAATGGGAGCAGTGGTGGAATGCCTTTAATGAGGAAAACCTGTTTTGCTCAGAAGAA
^ ^ ^ ^
Bsm Iw Mse I Dde I
Bsm I Mbo II
2770 2780 2790 2800 2810 2820
ATGCCATCTAGTGATGATGAGGCTACTGCTGACTCTCAACATTCTACTCCTCCAAAAAAG
^ ^ ^ ^
Mae I Hinf I Mnl I Mbo II
2830 2840 2850 2860 2870 2880
AAGAGAAAGGTAGAAGACCCCAAGGACTTTCCTTCAGAATTGTCAAGTTTTTTGAGTCAT
^ ^ ^ ^
Mbo II Ple I
Sty I Hinf I
Nla III
2890 2900 2910 2920 2930 2940
GCTGTGTTTAGTAATAGAACTCTTGCTTGCTTTGCTATTTACACCACAAAGGAAAAAGCT
^^
Alu I
Fnu4H I
2950 2960 2970 2980 2990 3000
GCACTGCTATACAAGAAAATTATGGAAAAATATTCTGTAACCTTTATAAGTAGGCATAAC
^ ^ ^
Ssp I Mae III Nsp I
3010 3020 3030 3040 3050 3060
AGTTATAATCATAACATACTGTTTTTTCTTACTCCACACAGGCATAGAGTGTCTGCTATT
^^
Ase I /Asn I
Mse I
3070 3080 3090 3100 3110 3120
AATAACTATGCTCAAAAATTGTGTACCTTTAGCTTTTTAATTTGTAAAGGGGTTAATAAG
^ ^ ^ ^
Rsa I Alu I Mse I
Mse I
3130 3140 3150 3160 3170 3180
GAATATTTGATGTATAGTGCCTTGACTAGAGATCATAATCAGCCATACCACATTTGTAGA
^ ^ ^
Ssp I Mae I
Dpn I
Mam I
Nde II
SauIIIA I
Mbo I
3190 3200 3210 3220 3230 3240
GGTTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAACCTGAAACATAAAATGAA
^^ ^ ^ ^
Dra I Mnl I Mnl I Bsm I
Mse I Bsm Iw
3250 3260 3270 3280 3290 3300
TGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAG
^^ ^ ^ ^ ^
Hind II Bbv I Mae III SfaN I
Hinc II Fnu4H I
Hpa I Alu I
Mse I
3310 3320 3330 3340 3350 3360
CATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAA
^
Mae I
3370 3380 3390 3400 3410 3420
ACTCATCAATGTATCTTATCATGTCTGGATCGGAATTCCGTGTATTCTATAGTGTCACCT
^ ^^ ^ ^
Nla III EcoR I Mae III
HinGU II
Alw I
Mbo I
SauIIIA I
Nde II
Dpn I
3430 3440 3450 3460 3470 3480
AAATCGTATGTGTATGATACATAAGGTTATGTATTAATTGTAGCCGCGTTCTAACGACAA
^^ ^ ^
Ase I /Asn I
Mse I Fnu4H I
Mvn I
Tha I
BstU I
FnuD II
Acc II
3490 3500 3510 3520 3530 3540
TATGTACAAGCCTAATTGTGTAGCATCTGGCTTACTGAAGCAGACCCTATCATCTCTCTC
^ ^
Rsa I SfaN I
3550 3560 3570 3580 3590 3600
GTAAACTGCCGTCAGAGTCGGTTTGGTTGGACGAACCTTCTGAGTTTCTGGTAACGCCGT
^ ^ ^ ^
Bbi II Dde I Mae III
HgiD I Hinf I
Aha II
Ple I
3610 3620 3630 3640 3650 3660
TCCGCACCCCGGAAATGGTCAGCGAACCAATCAGCAGGGTCATCGCTAGCCAGATCCTCT
^^ ^^ ^^ ^
Nci I Cel II Mnl I
Bcn I Nhe I Mbo I
ScrF I Mae I
Dsa V Xho II
Hap II BstY I
Msp I Mfl I
Hpa II SauIIIA I
Dpn I
Nde II
3670 3680 3690 3700 3710 3720
ACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATA
^ ^ ^ ^^^^ ^ ^ ^^ ^^
Msp I Eae I Msp I Nla IV
Hap II Hae III Hpa II Bbe I
Hpa II Pal I Hap II Nar I
Hga I Nae I Bbe I Acy I
SfaN I Msp I Nla IV Ban I
Hap II Ehe I Ehe I
Hpa II Ban I Hha I
SfaN I HinP I HinP I
Acy I Cfo I
Nar I
Cfo I
Hha I
3730 3740 3750 3760 3770 3780
TCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTT
^ ^ ^ ^^ ^^
Bbi II Mbo II BspH I
Nde II Nla III
SauIIIA I Hae II
Mbo I Eco 47 III
Dpn I Hha I
HinP I
Cfo I
3790 3800 3810 3820 3830 3840
TCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGC
^^ ^^ ^^^^ ^^ ^^
Eco0 109 I Nar I Bbu I
Pss I Eae I Acy I Sph I
Dra II Pal I Nla IV Nla III
Cfr I Hae III Ban I
Hae III Hap II Ehe I
Sau96 I ScrF I Bbe I
Asu I Cfr 10I Hha I
Pal I Hpa II Cfo I
Cfr 13I Dsa V HinP I
Nsp IV Bcn I
Nla IV Msp I
Nsp III
Dsa I
3850 3860 3870 3880 3890 3900
ATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCC
^ ^ ^ ^^ ^ ^ ^ ^
Fnu4H I Cfr I Bsr I EcoN I
Fnu4H I Eae IV Fnu4H I
Ban II Mnl I
HgiA I
Bsp 1286 I
Nsp II Pal I
Asp HI Hae III
3910 3920 3930 3940 3950 3960
TAATGCAGGAGTCGCATAAGGGAGAGCGTCGATATGGTGCACTCTCAGTACAATCTGCTC
^ ^ ^ ^ ^
Hinf I Taq I HgiA I Rsa I
Ple I Nsp II
Bsp 1286 I
Asp HI
Sno I
Apa LI
Dde I
3970 3980 3990 4000 4010 4020
TGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACG
^ ^ ^ ^^ ^
Fnu4H I Hga I Drd I
Mse I Hae II
BstU I
Acc II
FnuD II
Mvn I
Tha I
Nsp II
Ban II
Hha I
HinP I
Cfo I
Bsp 1286 I
4030 4040 4050 4060 4070 4080
GGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCAT
^^ ^ ^ ^ ^ ^ ^^ ^
Dsa V Alu I BsmA I Alu I
Nci I Mae III Bcn I Nla III
ScrF I ScrF I
Bcn I Hap II
Hpa II Hpa II
Hap II Dsa V
Msp I Msp I
SfaN I Fnu4H I
4090 4100 4110 4120 4130 4140
GTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACG
^^^ ^^^ ^^
FnuD II Pss I
BstU I Pal I
Tha I Dra II
Mvn I Eco0 109 I
Acc II Cfr 13I
HinP I Nsp IV
Hha I Sau96 I
Cfo I Asu I
Mvn I Hae III
Acc II Mnl I
FnuD II Nsp III
BstU I
Tha I
4150 4160 4170 4180 4190 4200
CCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTT
^ ^^ ^ ^^
Mse I Dde I
BspH I Acy I
Nla III HgiD I
Aat II
Aha II
Mea II
4210 4220 4230 4240 4250 4260
TCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTA
^^ ^
Hha I
Cfo I
HinP I
FnuD II
BstU I
Tha I
Acc II
Mvn I
Nla IV
4270 4280 4290 4300 4310 4320
TCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTAT
^^ ^ ^
BspH I Ssp I Mbo II
Nla III
4330 4340 4350 4360 4370 4380
GAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGT
^ ^
HgiD I Fnu4H I
Aha II
Bbi II
4390 4400 4410 4420 4430 4440
TTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACG
^ ^ ^ ^ ^
Hph I Mbo II HgiA I
Dpn I Asp HI
Mbo I Bsp 1286 I
Nde II Nsp II
SauIIIA I
Nsp I
Apa LI
Sno I
4450 4460 4470 4480 4490 4500
AGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGA
^ ^ ^ ^^ ^^ ^
Mae III Bsr I Xho II Mbo II
Taq I Nde II BstY I
HinGU II Mfl I
BstY I Nde II
Alw I SauIIIA I
Xho II Dpn I
Dpn I Mbo I
SauIIIA I
Mbo I
4510 4520 4530 4540 4550 4560
AGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCG
^ ^ ^ ^^ ^^
Asp 700 Bsp 1286 I Cfo I
Xmn I HgiA I HinP I
Mea II Asp HI Hha I
Nsp II BstU I
Dra I FnuD II
Mse I Tha I
Mvn I
Acc II
4570 4580 4590 4600 4610 4620
TATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGT
^ ^^ ^ ^ ^
Aha II Aha II Dde I
Hga I Bbi II
HgiD I HgiD I
Acy I Fnu4H I
Cfr 10I
ScrF I
Msp I
Hap II
Dsa V
Bcn I
Hpa II
4630 4640 4650 4660 4670 4680
TGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATG
^^ ^ ^ ^ ^ ^
Sca I Nsp I SfaN I Fok I
Rru I Mae III Nla III
Rsa I
4690 4700 4710 4720 4730 4740
CAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGG
^ ^ ^^^ ^^
Fnu4H I Nla III Fnu4H I Pvu I
Eae IV Mbo I
Eae I Nde II
Hae III SauIIIA I
Pal I Dpn I
4750 4760 4770 4780 4790 4800
AGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGA
^ ^ ^ ^^ ^ ^ ^
Cfr 13I Alu I Nla III Nla III Dpn I
Eco47 I HinGU II Mbo I
Nsp IV Alw I Nde II
Asu I SauIIIA I SauIIIA I
Sau96 I Mbo I
Ava II Nde II
Sin I Dpn I
Mae III
4810 4820 4830 4840 4850 4860
TCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCC
^ ^ ^ ^
Nla IV Mae III
Msp I
Hap II
Hpa II
Alu I
4870 4880 4890 4900 4910 4920
TGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTC
^ ^^ ^ ^ ^ ^ ^
Mea II Mse I Mae I
Mst I Bsr I Alu I
Aos I Bcn I
Avi II Nci I
Fsp I Dsa V
Fdi II ScrF I
Cfo I
HinP I
Hha I
4930 4940 4950 4960 4970 4980
CCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTC
^ ^^ ^ ^ ^ ^^ ^
Msp I Ase I /Asn I Sin I Hae II
Hpa II Mse I Fok I Eco47 I Bsp 1286 I
Hap II Bsr I Cfr 13I Cfo I
Ava II Ban II
Nsp IV HinP I
Sau96 I Nsp II
Asu I Hha I
Cfr I
Pss I
Eae IV
4990 5000 5010 5020 5030 5040
GGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCG
^^ ^ ^ ^^ ^ ^ ^ ^
Hae III Nla IV BsmA I
Pal I Cfr 10I Nsp III
Asu I Hap II Mvn I
Cfr 13I Msp I Tha I
Nsp IV Hpa II Acc II
Sau96 I Hph I FnuD II
Bgl I BstU I
Hpa II
Hap II
Msp I
5050 5060 5070 5080 5090 5100
CGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACAC
^ ^ ^^^ ^
Bbv I Nla IV Mnl I
Fnu4H I Hae III
Bsr I
Sau96 I
Asu I
Cfr 13I
Nsp IV
Pal I
5110 5120 5130 5140 5150 5160
GACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTC
^ ^ ^ ^ ^ ^
Hinf I Fok I Nde II Ban I
Ple I SauIIIA I Nla IV
Dpn I Mnl I
Mbo I
Dde I
5170 5180 5190 5200 5210 5220
ACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTT
^ ^ ^^
Mse I Mae III Dra I
Mse I
5230 5240 5250 5260 5270 5280
AAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGAC
^ ^^ ^^ ^ ^ ^ ^^ ^^
Mse I Alw I Mbo II BspH I
Dra I Mbo I BstY I Nla III
Mse I Mae I Mfl I
Xho II Xho II
HinGU II SauIIIA I
BstY I
Nde II Nde II
Dpn I Mbo I
SauIIIA I Dpn I
Hph I
5290 5300 5310 5320 5330 5340
CAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAA
^ ^ ^ ^
Mse I Dde I Mbo I
Mea II SauIIIA I
Nde II
Dpn I
5350 5360 5370 5380 5390 5400
AGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACC
^^ ^^ ^^ ^
Alw I Xho II HinP I Fnu4H I
HinGU II Mfl I Cfo I
BstY I Hha I
Xho II BstY I FnuD II
Dpn I Nde II Mvn I
SauIIIA I Tha I
Mbo I Dpn I BstU I
Nde II SauIIIA I Acc II
Mbo I
5410 5420 5430 5440 5450 5460
ACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGT
^ ^^ ^ ^
Hap II Alu I Mae III
Msp I
Hpa II
Alw I
HinGU II
Nde II
Mbo I
SauIIIA I
Dpn I
5470 5480 5490 5500 5510 5520
AACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGG
^ ^ ^ ^^
Bsr I Hha I Mae I Hae I
HinP I Hae III
Cfo I Pal I
5530 5540 5550 5560 5570 5580
CCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACC
^ ^ ^
Mnl I Mae III
Alw NI
5590 5600 5610 5620 5630 5640
AGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTT
^ ^ ^^ ^ ^
Fnu4H I Cfr 10I Mae III
Fnu4H I Msp I Hinf I
Hpa II
Hap II
Bcn I
Dsa V
ScrF I
5650 5660 5670 5680 5690 5700
ACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGA
^ ^ ^ ^ ^
Hpa II Cfo I Sno I Alu I
Msp I HinP I Nsp II
Hap II Hha I Apa LI
Bbv I Asp HI
Fnu4H I Bsp 1286 I
HgiA I
5710 5720 5730 5740 5750 5760
GCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCT
^ ^ ^^
Dde I Alu I Hae II
HinP I
Hha I
Cfo I
5770 5780 5790 5800 5810 5820
TCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCG
^ ^ ^
Hap II HinP I
Hpa II Nsp II
Msp I Bsp 1286 I
Fnu4H I Hha I
Cfo I
5830 5840 5850 5860 5870 5880
CACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCA
^ ^ ^
Alu I Dsa V
Dsa V EcoR II
ScrF I Apy I
EcoR II BstN I
BstN I ScrF I
Apy I Mva I
Mva I Nci I
Nci I
5890 5900 5910 5920 5930 5940
CCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAA
^ ^ ^ ^
Mnl I Taq I Nla IV
Drd I
5950 5960 5970 5980 5990 6000
CGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT
^^^^ ^ ^ ^^ ^^ ^^
Mvn I Nla IV Eae IV Afl III
BstU I Nci I Cfr I Nla III
FnuD II Dsa V Hae I
Tha I BstN I Pal I
Acc II Mva I Hae III
Fnu4H I Apy I
Eae IV ScrF I
Cfr I EcoR II
Hae III Eae IV
Pal I Hae I
Cfr I
Pal I
Hae III
6010 6020 6030 6040 6050 6060
CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGA
^ ^
Hinf I Alu I
6070 6080 6090 6100 6110 6120
TACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGA
^ ^ ^ ^ ^ ^ ^
Fnu4H I Cfo I Mbo II
Fnu4H I HinP I Hae II
Bbv I Hha I
Fnu4H I
Bbv I
Ple I
Hinf I
6130 6140 6150 6160 6170 6180
GCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCT
^ ^ ^ ^^^ ^^ ^ ^^ ^^^
Hha I Mnl I Mvn I Hae III Mse I
Bsp 1286 I Nsp III Pal I Ase I /Asn I
Cfo I BstU I Bbv I
HinP I FnuD II Hinf I Fnu4H I
Nsp II Acc II Pvu II
Ban II Tha I Alu I
Hha I
HinP I
Cfo I
Acc II
Tha I
Mvn I
FnuD II
BstU I
Eae I
6190 6200 6210
TATCGAAATTAATACGACTCACTATAGGGAGACCGG
^ ^^ ^ ^
Taq I Hinf I Msp I
Ase I /Asn I Hpa II
Mse I Hap II