Restriction map of Zd1 vector

        10        20        30        40        50        60               
AAGCTTGGGCTGCAGAAAAGACCCATGGAAAGGAACAGTCTGTTAGTCTGTCAGCTATTA               
^^      ^^            ^^          ^                 ^                      
Hind III              Dsa I       Nsp I             Alu I                  
 Alu I   Pst I        Nco I                                                
        Fnu4H I       Sty I                                                
                       Nla III                                             
        70        80        90       100       110       120               
TGTCTGGTGGCGCGCGCGGCAGCAACGAGTACTGCTCAGACTACACTGCCCTCCACCGTT               
         ^^^^^^^  ^        ^^     ^              ^       ^^                
         Cfo I    Fnu4H I   Rsa I                Mnl I   Hinc II           
         HinP I   Bbv I    Rru I  Dde I                  Hind II           
         BssH II           Sca I                         Hpa I             
         Hha I                                            Mse I            
          FnuD II                                                          
          BstU I                                                           
          Mvn I                                                            
          Tha I                                                            
          Acc II                                                           
           HinP I                                                          
           Cfo I                                                           
           BssH II                                                         
           Hha I                                                           
            BstU I                                                         
            Tha I                                                          
            FnuD II                                                        
            Acc II                                                         
            Mvn I                                                          
             Cfo I                                                         
             Hha I                                                         
             HinP I                                                        
              Acc II                                                       
              Tha I                                                        
              BstU I                                                       
              Mvn I                                                        
              FnuD II                                                      
               Fnu4H I                                                     
       130       140       150       160       170       180               
AACAGCACCGCAACGGGAGTTACCTCTGACTCTTATCAGAACACAACAACTCAGCTGCCT               
                  ^   ^    ^                     ^ ^^^                     
                  Mae III                        Dde I                     
                      Mnl I                        Pvu II                  
                           Hinf I                   Alu I                  
                                                     Fnu4H I               
       190       200       210       220       230       240               
GCATCTTCTTCTGCCGCTGCCTTAAGTCTTCCAAATGCGTCAGCGGTGCAAGCCCGCTCC               
^           ^  ^    ^^                                     ^               
SfaN I      Fnu4H I                                        Nsp III         
               Fnu4H I                                     Ava I           
                    Afl II                                                 
                    Bfr I                                                  
                     Mse I                                                 
       250       260       270       280       290       300               
CCGAGCTCATTTTCAGACACATACCCTACCGCCACGGCCTTGTGCGGCACACTGGTGGTG               
  ^^                           ^  ^^       ^      ^                        
  Asp HI                       Dsa I       Fnu4H I                         
  Ban II                          Cfr I           Bsr I                    
  Nsp II                          Eae IV                                   
  HgiA I                           Pal I                                   
  Bsp 1286 I                       Hae III                                 
  Sst I                                                                    
  Sac I                                                                    
   Alu I                                                                   
       310       320       330       340       350       360               
GTGGGCATCGTGCTGTGCCTAAGTCTGGCCTCCACTGTTAGGAGCAAGGAGCTGCCGAGC               
    ^             ^      ^^ ^                    ^^                        
    SfaN I        Dde I     Mnl I                Alu I                     
                         Eae IV                   Fnu4H I                  
                         Hae I                                             
                         Cfr I                                             
                          Hae III                                          
                          Pal I                                            
       370       380       390       400       410       420               
GACCATGAGTCGCTGGAGGCATGGGAGCAGGGCTCGGATGTAGAAGCTCCGCCGCTACCG               
   ^  ^            ^               ^        ^     ^      ^                 
   Nla III         Nla III         Fok I    Alu I        Hap II            
      Hinf I                                      Fnu4H I                  
      Ple I                                              Msp I             
                                                         Hpa II            
       430       440       450       460       470       480               
GAGAAGAGCCCATGTCCGGAACACGTACCCGAGATTCGCGTGGAGATCCCACGTTATGTT               
  ^  ^    ^   ^^    ^^^ ^  ^    ^   ^      ^^     ^        ^               
  Mbo II      BspM II   Rsa I   Hinf I     Xho II          Mse I           
     Nsp II   Mro I        Ava I    Tha I   Nde II                         
     Bsp 1286 I     Afl III         BstU I                                 
     Ban II    Hpa II      Nsp III         BstY I                          
          Nla III    Bsa I          Mvn I   SauIIIA I                      
              Acc III               FnuD II       Mea II                   
               Msp I                Acc II                                 
               Hap II                      Mfl I                           
                      Mea II                Dpn I                          
                                            Mbo I                          
       490       500       510       520       530       540               
TAATAAAAACTGCGGGCACTGGGGACGGTGGTGTTGTATATGTGAATTTGTAAATAATAA               
                 ^                                                         
                 Bsr I                                                     
       550       560       570       580       590       600               
ATGAGACCCCATCCTGTAAAAATACAGAGTCCGTGTCAGTCTCTGAAGGACAGTGTATTG               
                          ^        ^^ ^                                    
                          Ple I    Nsp I                                   
                          Hinf I    Alw NI                                 
                                      BsmA I                               
       610       620       630       640       650       660               
GCATATAGCCAATAAAGAGAGTTGTGGCAAAGAGCCATGTTATGGATTAGTAATGGAAAG               
                                  ^^                                       
                                  PflM I                                   
                                   Nla III                                 
       670       680       690       700       710       720               
TATCGTCACCAATAGGGGAGTGGTCAATAATGGTCAATAACCCACACCTATAGGCTAAGC               
    ^   ^                                            ^^  ^                 
    Mae III                                          Esp I                 
        Xcm I                                         Dde I                
                                                         Alu I             
       730       740       750       760       770       780               
TATACCATCACCTATAACATGAGGAAGCGGGGGTGTATAGACCCCAAGCCAAAAACAGTA               
                 ^                                                         
                 Nla III                                                   
       790       800       810       820       830       840               
TAGCATGCATAAGAAGCCAAGGGGGTGGGCCTATAGACTCTATAGGCGGTACTTACGTCA               
  ^^^           ^        ^^^       ^            ^     ^ ^                  
  Bbu I         BstX I    Sau96 I               Rsa I   Mae III            
  Sph I         Sty I    Pss I     Hinf I             Mea II               
   Nla III                Cfr 13I                                          
    EcoT22 I              Asu I                                            
    Nsi I                 Nsp IV                                           
                           Pal I                                           
                           Hae III                                         
       850       860       870       880       890       900               
CTCTTGGCACGGGGAATCCGCGTTCCAATGCACCGTTCCCGGCCGCGGAGGCTGGATCGG               
             ^    ^                  ^^^^^^^         ^^  ^^                
             Hinf I                  Bcn I           HinGU II              
                  FnuD II            Nci I           Alw I                 
                  Acc II             ScrF I           Mbo I                
                  Tha I              Dsa V            Nde II               
                  BstU I              Msp I           Dpn I                
                  Mvn I               Hpa II          SauIIIA I            
                                      Hap II             Pss I             
                                       Eae I             PpuM I            
                                       Ecl X I            Eco47 I          
                                       Eag I              Ava II           
                                       Xma III            Nla IV           
                                       Eco52 I            Asu I            
                                        Pal I             Nsp IV           
                                        Hae III           Sau96 I          
                                         Fnu4H I          Cfr 13I          
                                         Bgl I            Sin I            
                                          Ksp I                            
                                          Sst II                           
                                          Dsa I                            
                                          Sac II                           
                                           BstU I                          
                                           Mvn I                           
                                           Acc II                          
                                           FnuD II                         
                                           Tha I                           
       910       920       930       940       950       960               
TCCCGGTGTCTTCTATGGAGGTCAAAACAGCGTGGATGGCGTCTCCAGGCGATCTGACGG               
 ^^                              ^   ^  ^   ^    ^^                        
 Nci I                           Fok I      Apy I                          
 ScrF I                              Acy I       Mfl I                     
 Dsa V                                  BsmA I    Dpn I                    
 Bcn I                                      Mva I                          
  Msp I                                     BstN I                         
  Hpa II                                    EcoR II                        
  Hap II                                    ScrF I                         
                                            Dsa V                          
                                            Nci I                          
                                                  SauIIIA I                
                                                  Mbo I                    
                                                  Nde II                   
       970       980       990      1000      1010      1020               
TTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGC               
          ^^                ^        ^                                     
          Nsp II            Mnl I    Rsa I                                 
          Ban II                                                           
          Sst I                                                            
          Sac I                                                            
          Asp HI                                                           
          HgiA I                                                           
          Bsp 1286 I                                                       
           Alu I                                                           
      1030      1040      1050      1060      1070      1080               
GTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAAC               
                 ^                               ^                         
                 Mae III                         Ban I                     
                                                 Nla IV                    
      1090      1100      1110      1120      1130      1140               
AAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCGCTAT               
           ^^                           ^    ^                             
           Aha II                       Nsp III                            
           Aat II                            Hinf I                        
           HgiD I                            Ple I                         
           Acy I                                                           
            Mea II                                                         
      1150      1160      1170      1180      1190      1200               
CCACGCCCATTGATGTACTGCCAAAACCGCATCACCATGGTAATAGCGATGACTAATACG               
              ^             ^     ^^                    ^^                 
              Rsa I         SfaN I                      Bsa I              
                                  Dsa I                 Eco105 I           
                                  Sty I                 SnaB I             
                                  Nco I                  Mea II            
                                   Nla III                                 
      1210      1220      1230      1240      1250      1260               
TAGATGTACTGCCAAGTAGGAAAGTCCCATAAGGTCATGTACTGGGCATAATGCCAGGCG               
     ^                             ^  ^ ^           ^^     ^               
     Rsa I                         Nla III          Bgl I                  
                                      Rsa I          Nci I                 
                                        Bsr I        ScrF I                
                                                     BstN I                
                                                     Apy I                 
                                                     Dsa V                 
                                                     EcoR II               
                                                     Mva I                 
                                                           Asu I           
                                                           Sau96 I         
                                                           Nsp IV          
                                                           Cfr 13I         
      1270      1280      1290      1300      1310      1320               
GGCCATTTACCGTCATTGACGTCAATAGGGGGCGTACTTGGCATATGATACACTTGATGT               
^                ^^              ^       ^                ^                
Hae III          Aat II          Rsa I   Nde I            Rsa I            
Pal I            Acy I                                                     
                 Aha II                                                    
                 HgiD I                                                    
                  Mea II                                                   
      1330      1340      1350      1360      1370      1380               
ACTGCCAAGTGGGCAGTTTACCGTAAATACTCCACCCATTGACGTCAATGGAAAGTCCCT               
   ^                                    ^^                                 
   Bgl I                                Aha II                             
                                        Acy I                              
                                        Aat II                             
                                        HgiD I                             
                                         Mea II                            
      1390      1400      1410      1420      1430      1440               
ATTGGCGTTACTATGGGAACATACGTCATTATTGACGTCAATGGGCGGGGGTCGTTGGGC               
      ^               ^          ^^                                        
      Mae III         Mea II     Aha II                                    
                                 HgiD I                                    
                                 Aat II                                    
                                 Acy I                                     
                                  Mea II                                   
      1450      1460      1470      1480      1490      1500               
GGTCAGCCAGGCGGGCCATTTACCGTAAGTTATGTAACGCGGAACTCCATATATGGGCTA               
     ^^     ^^                   ^   ^                                     
     Bgl I   Hae III             Mae III                                   
      Mva I                          Acc II                                
      Apy I                          FnuD II                               
      Nci I                          Tha I                                 
      BstN I                         Mvn I                                 
      ScrF I                         BstU I                                
      Dsa V                                                                
      EcoR II                                                              
            Sau96 I                                                        
            Nsp IV                                                         
            Asu I                                                          
            Cfr 13I                                                        
             Pal I                                                         
      1510      1520      1530      1540      1550      1560               
TGAACTAATGACCCCGTAATTGATTACTATTAATAACTAGTCAATAATCAATGTCAACAT               
                            ^^     ^^               ^    ^                 
                            Ase I   /Asn I          Hind II                
                             Mse I                  Hinc II                
                                   Spe I                 Nla III           
                                    Mae I                                  
      1570      1580      1590      1600      1610      1620               
GGCGGTAATGTTGGACATGAGCCAATATAAATGTACATATTATGATATGGATACAACGTA               
               ^                ^                      ^                   
               Nla III          Rsa I                  Mea II              
      1630      1640      1650      1660      1670      1680               
TGCAATGGCCAATAGCCAATATTGATTTATGCTATATAACCAATGAATAATATGGCTAAT               
     ^^          ^                                         ^               
     Eae IV      Ssp I                                     Hae I           
     Hae I                                                 Eae IV          
     Eae I                                                 Eae I           
     Bal I                                                 Bal I           
      Pal I                                                                
      Hae III                                                              
      1690      1700      1710      1720      1730      1740               
GGCCAATATTGATTCAATGTATAGATCGATATGCATTGGCCATGTGCCAGCTTGATGTCG               
^   ^     ^            ^^^    ^     ^^  ^       ^       ^                  
Pal I     Hinf I       Mbo I        Bal I       Alu I   Aha II             
Hae III                Nde II       Eae IV              HgiD I             
    Ssp I              SauIIIA I    Eae I               Bbi II             
                       Dpn I        Hae I                                  
                        Cla I        Pal I                                 
                        Ban III      Hae III                               
                         Taq I          Nla III                            
                              Nsi I                                        
                              EcoT22 I                                     
      1750      1760      1770      1780      1790      1800               
CCTCTATCGGCGATATAGCCTCATATCGTCTGTCACCTATATCGAAACTGCGATATTTGC               
^                 ^            ^         ^                                 
Mnl I             Mnl I        Mae III   Taq I                             
      1810      1820      1830      1840      1850      1860               
GACACACAGAATCGCCCAAGTCACCAAAGTCGTCTATCGCCATCCCCCGTAAACGATATA               
        ^          ^        ^                                              
        Hinf I     Mae III                                                 
                            Bbi II                                         
                            Aha II                                         
                            HgiD I                                         
      1870      1880      1890      1900      1910      1920               
AGCCGCTATCGCCAGATATCGCGTATGCCCAAAAATCACTTTTGGAAAAATGGCGATATC               
 ^            ^    ^                                  ^   ^                
 Fnu4H I      EcoR V                                  EcoR V               
                   BstU I                                 Nsp I            
                   Acc II                                                  
                   Mvn I                                                   
                   FnuD II                                                 
                   Tha I                                                   
      1930      1940      1950      1960      1970      1980               
AGTTACACAGAAACTCACATCGGCGACATTTTCAATATGCCATATTTTCAAATATCGATT               
 ^                                                   ^^                    
 Mae III                                             Cla I                 
                                                     Ban III               
                                                      Taq I                
      1990      2000      2010      2020      2030      2040               
TTTCCAATATCGCCATCTCTATCGGCGATAAACACCACTATCGCGCGACATGAATTTAGT               
                                         ^^^    ^                          
                                         Mvn I                             
                                         BstU I                            
                                         FnuD II                           
                                         Tha I                             
                                         Acc II                            
                                          HinP I                           
                                          Hha I                            
                                          Cfo I                            
                                           Acc II                          
                                           Tha I                           
                                           BstU I                          
                                           Mvn I                           
                                           FnuD II                         
                                                Nla III                    
      2050      2060      2070      2080      2090      2100               
CGGCGACAGAAATCTCAAAACGCGTATTTCGGACAAACACACATTTTATTATTCACTGCA               
                   ^^                                  ^                   
                   Mlu I                               Pst I               
                   Afl III                                                 
                    BstU I                                                 
                    Mvn I                                                  
                    FnuD II                                                
                    Tha I                                                  
                    Acc II                                                 
      2110      2120      2130      2140      2150      2160               
GGTCGATCGACTCTAGCTAGAGCGGGCCACCTTTGCTATCTTTGCTGAAGATGATGGACC               
  ^^^ ^ ^   ^ ^ ^      ^^                     ^       ^^                   
  REM I     Mae I      Sau96 I                Mbo II   Sau96 I             
  Taq I       Alu I    Asu I                          Pss I                
   Pvu I        Mae I   Pal I                         PpuM I               
    Dpn I              Nsp IV                          Sin I               
    Mbo I              Cfr 13I                         Eco47 I             
    SauIIIA I           Hae III                        Asu I               
    Nde II                                             Cfr 13I             
      Taq I                                            Nla IV              
        Hinf I                                         Nsp IV              
                                                       Ava II              
      2170      2180      2190      2200      2210      2220               
CAAACTCGACTTCTGAAGATGTAAAATTTACACCTGACCCATACCAGGTGCCTTTTGTAC               
     ^        ^                            ^  ^         ^                  
     Taq I    Mbo II                       Mva I        Rsa I              
                                           Nci I                           
                                           BstN I                          
                                           EcoR II                         
                                           Apy I                           
                                           ScrF I                          
                                           Dsa V                           
                                              Nla IV                       
                                              Ban I                        
      2230      2240      2250      2260      2270      2280               
AAGCTCTAGCAGACATTGGTGTTCCACAGCCTGCACCAGTGGCTGCCCCGGCACGACGCA               
 ^   ^                              ^    ^    ^^      ^                    
 Alu I                              Alw NI    Nci I   Hga I                
     Mae I                               Fnu4H I                           
                                              Bcn I                        
                                              ScrF I                       
                                              Dsa V                        
                                               Msp I                       
                                               Hap II                      
                                               Hpa II                      
      2290      2300      2310      2320      2330      2340               
CACGGAAACCACAACAGCCAGAATCGCTGGAGGAATGCGATTCTGAACTAGAAATAAAGC               
                    ^           ^     ^        ^                           
                    Hinf I      Bsm IwHinf I   Mae I                       
                                Bsm I                                      
      2350      2360      2370      2380      2390      2400               
GATACAAGAATCGGGTGGCTTCCAGAAAATGCCGGGCCAAGTTTAAGCAACTGCTGCAGC               
       ^                      ^^ ^^       ^         ^^ ^                   
       Hinf I                 Cfr 10I     Mse I     Fnu4H I                
                               Msp I                 Pst I                 
                               Bcn I                   Bbv I               
                               ScrF I                  Fnu4H I             
                               Dsa V                                       
                               Hpa II                                      
                               Hap II                                      
                                 Sau96 I                                   
                                 Asu I                                     
                                 Nsp IV                                    
                                 Cfr 13I                                   
                                  Hae III                                  
                                  Pal I                                    
      2410      2420      2430      2440      2450      2460               
ACTACCGTGAGGTGGCTGCTGCCAAATCATCTGAAAATGACAGGCTGCGCCTCCTGTTGA               
              ^  ^                         ^ ^^  ^                         
              Fnu4H I                      Fnu4H I                         
                 Fnu4H I                     Hae II                        
                                              Hha I                        
                                              HinP I                       
                                              Cfo I                        
                                                 Mnl I                     
      2470      2480      2490      2500      2510      2520               
AGCAGATGTGCCCAAGCCTGGATGTTGCTCTAGAGGATCCCCGGGGTCGACCTCGAGCCT               
       ^        ^  ^        ^^    ^^   ^^    ^^   ^^^     ^                
       Nsp II   Mva I       Xba I      Xma I      Mnl I   Mae I            
       Bsp 1286 I            Mae I     Dsa V       Nsp III                 
       Ban II      Fok I          Bst I      Hind II                       
                ScrF I            Alw I      Hinc II                       
                BstN I            Xho II     Bbi II                        
                Apy I             BstY I           PaeR7 I                 
                Dsa V             HinGU II   Sal I                         
                EcoR II           Nla IV     Acc I                         
                Nci I             BamH I      Taq I                        
                                   SauIIIA I       Xho I                   
                                   Mbo I           Ava I                   
                                   Dpn I            Taq I                  
                                   Nde II                                  
                                       Bcn I                               
                                       ScrF I                              
                                       Sma I                               
                                       Ava I                               
                                       Xcy I                               
                                       Nci I                               
                                        Hpa II                             
                                        Dsa V                              
                                        Msp I                              
                                        Bcn I                              
                                        Hap II                             
                                        ScrF I                             
      2530      2540      2550      2560      2570      2580               
AGCTAGCTAGGCTCGAGGGATCTTTGTGAAGGAACCTTACTTCTGTGGTGTGACATAATT               
^^^ ^ ^    ^^    ^^           ^                  ^                         
Alu I      Ava I              Nla IV             Mae III                   
 Nhe I     PaeR7 I                                                         
 Cel II    Nsp III                                                         
  Mae I    Xho I                                                           
    Alu I   Taq I                                                          
      Mae I      Alw I                                                     
                 BstY I                                                    
                 Xho II                                                    
                 HinGU II                                                  
                  Dpn I                                                    
                  Nde II                                                   
                  Mbo I                                                    
                  SauIIIA I                                                
      2590      2600      2610      2620      2630      2640               
GGACAAACTACCTACAGAGATTTAAAGCTCTAAGGTAAATATAAAATTTTTAAGTGTATA               
                    ^^   ^   ^                   ^                         
                    Dra I    Dde I               Mse I                     
                     Mse I                                                 
                         Alu I                                             
      2650      2660      2670      2680      2690      2700               
ATGTGTTAAACTACTGATTCTAATTGTTTGTGTATTTTAGATTCCAACCTATGGAACTGA               
     ^         ^                       ^   ^                               
     Mse I     Hinf I                  Hinf I                              
                                           PflM I                          
      2710      2720      2730      2740      2750      2760               
TGAATGGGAGCAGTGGTGGAATGCCTTTAATGAGGAAAACCTGTTTTGCTCAGAAGAAAT               
                  ^       ^                     ^   ^                      
                  Bsm Iw  Mse I                 Dde I                      
                  Bsm I                             Mbo II                 
      2770      2780      2790      2800      2810      2820               
GCCATCTAGTGATGATGAGGCTACTGCTGACTCTCAACATTCTACTCCTCCAAAAAAGAA               
     ^                      ^                 ^          ^                 
     Mae I                  Hinf I            Mnl I      Mbo II            
      2830      2840      2850      2860      2870      2880               
GAGAAAGGTAGAAGACCCCAAGGACTTTCCTTCAGAATTGTCAAGTTTTTTGAGTCATGC               
          ^      ^                                 ^   ^                   
          Mbo II                                   Ple I                   
                 Sty I                             Hinf I                  
                                                       Nla III             
      2890      2900      2910      2920      2930      2940               
TGTGTTTAGTAATAGAACTCTTGCTTGCTTTGCTATTTACACCACAAAGGAAAAAGCTGC               
                                                      ^^                   
                                                      Alu I                
                                                       Fnu4H I             
      2950      2960      2970      2980      2990      3000               
ACTGCTATACAAGAAAATTATGGAAAAATATTCTGTAACCTTTATAAGTAGGCATAACAG               
                          ^       ^                     ^                  
                          Ssp I   Mae III               Nsp I              
      3010      3020      3030      3040      3050      3060               
TTATAATCATAACATACTGTTTTTTCTTACTCCACACAGGCATAGAGTGTCTGCTATTAA               
                                                       ^^                  
                                                       Ase I   /Asn I      
                                                        Mse I              
      3070      3080      3090      3100      3110      3120               
TAACTATGCTCAAAAATTGTGTACCTTTAGCTTTTTAATTTGTAAAGGGGTTAATAAGGA               
                    ^       ^     ^               ^        ^               
                    Rsa I   Alu I                 Mse I    Ssp I           
                                  Mse I                                    
      3130      3140      3150      3160      3170      3180               
ATATTTGATGTATAGTGCCTTGACTAGAGATCATAATCAGCCATACCACATTTGTAGAGG               
                       ^    ^                                              
                       Mae I                                               
                            Dpn I                                          
                            Mam I                                          
                            Nde II                                         
                            SauIIIA I                                      
                            Mbo I                                          
      3190      3200      3210      3220      3230      3240               
TTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAACCTGAAACATAAAATGAATG               
          ^^       ^        ^                          ^                   
          Dra I    Mnl I    Mnl I                      Bsm I               
           Mse I                                       Bsm Iw              
      3250      3260      3270      3280      3290      3300               
CAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCA               
           ^^             ^ ^          ^                 ^                 
           Hind II        Bbv I        Mae III           SfaN I            
           Hinc II        Fnu4H I                                          
           Hpa I            Alu I                                          
            Mse I                                                          
      3310      3320      3330      3340      3350      3360               
TCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAAC               
                                       ^                                   
                                       Mae I                               
      3370      3380      3390      3400      3410      3420               
TCATCAATGTATCTTATCATGTCTGGATCGGAATTCCGTGTATTCTATAGTGTCACCTAA               
                 ^      ^^    ^                    ^                       
                 Nla III      EcoR I               Mae III                 
                        HinGU II                                           
                        Alw I                                              
                         Mbo I                                             
                         SauIIIA I                                         
                         Nde II                                            
                         Dpn I                                             
      3430      3440      3450      3460      3470      3480               
ATCGTATGTGTATGATACATAAGGTTATGTATTAATTGTAGCCGCGTTCTAACGACAATA               
                              ^^        ^ ^                                
                              Ase I   /Asn I                               
                               Mse I    Fnu4H I                            
                                          Mvn I                            
                                          Tha I                            
                                          BstU I                           
                                          FnuD II                          
                                          Acc II                           
      3490      3500      3510      3520      3530      3540               
TGTACAAGCCTAATTGTGTAGCATCTGGCTTACTGAAGCAGACCCTATCATCTCTCTCGT               
 ^                  ^                                                      
 Rsa I              SfaN I                                                 
      3550      3560      3570      3580      3590      3600               
AAACTGCCGTCAGAGTCGGTTTGGTTGGACGAACCTTCTGAGTTTCTGGTAACGCCGTTC               
     ^      ^                        ^          ^                          
     Bbi II                          Dde I      Mae III                    
     HgiD I Hinf I                                                         
     Aha II                                                                
            Ple I                                                          
      3610      3620      3630      3640      3650      3660               
CGCACCCCGGAAATGGTCAGCGAACCAATCAGCAGGGTCATCGCTAGCCAGATCCTCTAC               
     ^^                                   ^^     ^^  ^                     
     Nci I                                Cel II     Mnl I                 
     Bcn I                                Nhe I   Mbo I                    
     ScrF I                                Mae I                           
     Dsa V                                       Xho II                    
      Hap II                                     BstY I                    
      Msp I                                      Mfl I                     
      Hpa II                                      SauIIIA I                
                                                  Dpn I                    
                                                  Nde II                   
      3670      3680      3690      3700      3710      3720               
GCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATC               
 ^  ^  ^     ^^^^  ^     ^ ^^                   ^^                         
 Msp I       Eae I       Msp I                  Nla IV                     
 Hap II       Hae III    Hpa II                 Bbe I                      
 Hpa II       Pal I      Hap II                 Nar I                      
    Hga I      Nae I       Bbe I                Acy I                      
       SfaN I   Msp I      Nla IV               Ban I                      
                Hap II     Ehe I                Ehe I                      
                Hpa II     Ban I                 Hha I                     
                   SfaN I   HinP I               HinP I                    
                           Acy I                 Cfo I                     
                           Nar I                                           
                            Cfo I                                          
                            Hha I                                          
      3730      3740      3750      3760      3770      3780               
GCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTC               
^                 ^  ^                     ^^   ^^                         
Bbi II            Mbo II                   BspH I                          
                     Nde II                 Nla III                        
                     SauIIIA I                  Hae II                     
                     Mbo I                      Eco 47 III                 
                     Dpn I                       Hha I                     
                                                 HinP I                    
                                                 Cfo I                     
      3790      3800      3810      3820      3830      3840               
GGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCAT               
                 ^^  ^^  ^^^^             ^^            ^^                 
                 Eco0 109 I               Nar I         Bbu I              
                 Pss I   Eae I            Acy I         Sph I              
                 Dra II   Pal I           Nla IV         Nla III           
                 Cfr I    Hae III         Ban I                            
                  Hae III   Hap II        Ehe I                            
                  Sau96 I   ScrF I        Bbe I                            
                  Asu I    Cfr 10I         Hha I                           
                  Pal I     Hpa II         Cfo I                           
                  Cfr 13I   Dsa V          HinP I                          
                  Nsp IV    Bcn I                                          
                  Nla IV    Msp I                                          
                     Nsp III                                               
                      Dsa I                                                
      3850      3860      3870      3880      3890      3900               
GCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTA               
            ^  ^     ^       ^^ ^           ^    ^      ^                  
            Fnu4H I          Cfr I          Bsr I       EcoN I             
               Fnu4H I       Eae IV              Fnu4H I                   
                     Ban II     Mnl I                                      
                     HgiA I                                                
                     Bsp 1286 I                                            
                     Nsp II   Pal I                                        
                     Asp HI   Hae III                                      
      3910      3920      3930      3940      3950      3960               
ATGCAGGAGTCGCATAAGGGAGAGCGTCGATATGGTGCACTCTCAGTACAATCTGCTCTG               
      ^                   ^       ^      ^   ^                             
      Hinf I              Taq I   HgiA I     Rsa I                         
      Ple I                       Nsp II                                   
                                  Bsp 1286 I                               
                                  Asp HI                                   
                                  Sno I                                    
                                  Apa LI                                   
                                         Dde I                             
      3970      3980      3990      4000      4010      4020               
ATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGG               
  ^        ^                                ^ ^^      ^                    
  Fnu4H I                                   Hga I     Drd I                
           Mse I                              Hae II                       
                                              BstU I                       
                                              Acc II                       
                                              FnuD II                      
                                              Mvn I                        
                                              Tha I                        
                                               Nsp II                      
                                               Ban II                      
                                               Hha I                       
                                               HinP I                      
                                               Cfo I                       
                                               Bsp 1286 I                  
      4030      4040      4050      4060      4070      4080               
CTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGT               
          ^^  ^                ^   ^     ^   ^    ^^   ^                   
          Dsa V                Alu I     BsmA I   Alu I                    
          Nci I                    Mae III   Bcn I     Nla III             
          ScrF I                             ScrF I                        
          Bcn I                              Hap II                        
           Hpa II                            Hpa II                        
           Hap II                            Dsa V                         
           Msp I                             Msp I                         
              SfaN I                               Fnu4H I                 
      4090      4100      4110      4120      4130      4140               
GTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCC               
                             ^^^          ^^^ ^^                           
                             FnuD II      Pss I                            
                             BstU I         Pal I                          
                             Tha I        Dra II                           
                             Mvn I        Eco0 109 I                       
                             Acc II        Cfr 13I                         
                              HinP I       Nsp IV                          
                              Hha I        Sau96 I                         
                              Cfo I        Asu I                           
                               Mvn I        Hae III                        
                               Acc II         Mnl I                        
                               FnuD II         Nsp III                     
                               BstU I                                      
                               Tha I                                       
      4150      4160      4170      4180      4190      4200               
TATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTC               
            ^     ^^                ^   ^^                                 
            Mse I                   Dde I                                  
                  BspH I                Acy I                              
                   Nla III              HgiD I                             
                                        Aat II                             
                                        Aha II                             
                                         Mea II                            
      4210      4220      4230      4240      4250      4260               
GGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATC               
          ^^  ^                                                            
          Hha I                                                            
          Cfo I                                                            
          HinP I                                                           
           FnuD II                                                         
           BstU I                                                          
           Tha I                                                           
           Acc II                                                          
           Mvn I                                                           
              Nla IV                                                       
      4270      4280      4290      4300      4310      4320               
CGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGA               
   ^^                               ^            ^                         
   BspH I                           Ssp I        Mbo II                    
    Nla III                                                                
      4330      4340      4350      4360      4370      4380               
GTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTT               
                 ^                    ^                                    
                 HgiD I               Fnu4H I                              
                 Aha II                                                    
                 Bbi II                                                    
      4390      4400      4410      4420      4430      4440               
TTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAG               
                   ^                  ^  ^ ^       ^                       
                   Hph I              Mbo II       HgiA I                  
                                         Dpn I     Asp HI                  
                                         Mbo I     Bsp 1286 I              
                                         Nde II    Nsp II                  
                                         SauIIIA I                         
                                           Nsp I                           
                                                   Apa LI                  
                                                   Sno I                   
      4450      4460      4470      4480      4490      4500               
TGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAG               
   ^     ^   ^  ^^               ^^                     ^  ^               
   Mae III   Bsr I               Xho II                 Mbo II             
         Taq I   Nde II          BstY I                    Asp 700         
                HinGU II         Mfl I                     Xmn I           
                BstY I            Nde II                                   
                Alw I             SauIIIA I                                
                Xho II            Dpn I                                    
                 Dpn I            Mbo I                                    
                 SauIIIA I                                                 
                 Mbo I                                                     
      4510      4520      4530      4540      4550      4560               
AACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTA               
 ^              ^      ^^                 ^^                               
 Mea II         Bsp 1286 I                Cfo I                            
                HgiA I                    HinP I                           
                Asp HI                    Hha I                            
                Nsp II                     BstU I                          
                       Dra I               FnuD II                         
                        Mse I              Tha I                           
                                           Mvn I                           
                                           Acc II                          
      4570      4580      4590      4600      4610      4620               
TTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTG               
  ^  ^^                 ^  ^              ^                                
  Aha II                Aha II            Dde I                            
  Hga I                 Bbi II                                             
  HgiD I                HgiD I                                             
  Acy I                    Fnu4H I                                         
     Cfr 10I                                                               
      ScrF I                                                               
      Msp I                                                                
      Hap II                                                               
      Dsa V                                                                
      Bcn I                                                                
      Hpa II                                                               
      4630      4640      4650      4660      4670      4680               
AGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCA               
^^      ^  ^          ^        ^     ^                                     
Sca I   Nsp I         SfaN I   Fok I                                       
Rru I      Mae III                   Nla III                               
 Rsa I                                                                     
      4690      4700      4710      4720      4730      4740               
GTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAG               
  ^          ^               ^^^                   ^^      ^               
  Fnu4H I    Nla III         Fnu4H I               Pvu I   Cfr 13I         
                              Eae IV                Mbo I                  
                              Eae I                 Nde II                 
                               Hae III              SauIIIA I              
                               Pal I                Dpn I                  
                                                           Eco47 I         
                                                           Nsp IV          
                                                           Asu I           
                                                           Sau96 I         
                                                           Ava II          
                                                           Sin I           
      4750      4760      4770      4780      4790      4800               
GACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATC               
         ^                     ^     ^^  ^  ^           ^                  
         Alu I                 Nla III   Nla III        Dpn I              
                                     HinGU II           Mbo I              
                                     Alw I              Nde II             
                                      SauIIIA I         SauIIIA I          
                                      Mbo I                                
                                      Nde II                               
                                      Dpn I                                
                                            Mae III                        
      4810      4820      4830      4840      4850      4860               
GTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTG               
    ^   ^   ^                             ^                                
    Nla IV                                Mae III                          
        Msp I                                                              
        Hap II                                                             
        Hpa II                                                             
            Alu I                                                          
      4870      4880      4890      4900      4910      4920               
TAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCC               
              ^   ^^         ^  ^                 ^ ^    ^^                
              Mea II         Mse I                Mae I   Msp I            
                  Mst I         Bsr I               Alu I                  
                  Aos I                                  Bcn I             
                  Avi II                                 Nci I             
                  Fsp I                                  Dsa V             
                  Fdi II                                 ScrF I            
                   Cfo I                                  Hpa II           
                   HinP I                                 Hap II           
                   Hha I                                                   
      4930      4940      4950      4960      4970      4980               
GGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGG               
       ^^      ^  ^                      ^         ^^    ^^^               
       Ase I   /Asn I                    Sin I     Hae II                  
        Mse I     Fok I                  Eco47 I    Bsp 1286 I             
               Bsr I                     Cfr 13I    Cfo I                  
                                         Ava II     Ban II                 
                                         Nsp IV     HinP I                 
                                         Sau96 I    Nsp II                 
                                         Asu I      Hha I                  
                                                         Cfr I             
                                                         Pss I             
                                                         Eae IV            
                                                          Hae III          
                                                          Pal I            
                                                          Asu I            
                                                          Cfr 13I          
                                                          Nsp IV           
                                                          Sau96 I          
                                                           Bgl I           
      4990      5000      5010      5020      5030      5040               
CCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCG               
     ^                             ^  ^^ ^          ^ ^ ^                  
     Hpa II                        Nla IV           BsmA I                 
     Hap II                           Cfr 10I         Nsp III              
     Msp I                             Hap II           Mvn I              
                                       Msp I            Tha I              
                                       Hpa II           Acc II             
                                         Hph I          FnuD II            
                                                        BstU I             
      5050      5060      5070      5080      5090      5100               
GTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGA               
        ^    ^  ^^^              ^                                         
        Bbv I   Nla IV           Mnl I                                     
        Fnu4H I   Hae III                                                  
             Bsr I                                                         
                 Sau96 I                                                   
                 Asu I                                                     
                 Cfr 13I                                                   
                 Nsp IV                                                    
                  Pal I                                                    
      5110      5120      5130      5140      5150      5160               
CGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCAC               
    ^              ^                 ^    ^       ^   ^                    
    Hinf I         Fok I             Nde II       Ban I                    
    Ple I                            SauIIIA I    Nla IV                   
                                     Dpn I            Mnl I                
                                     Mbo I                                 
                                          Dde I                            
      5170      5180      5190      5200      5210      5220               
TGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAA               
   ^         ^                                         ^^                  
   Mse I     Mae III                                   Dra I               
                                                        Mse I              
      5230      5240      5250      5260      5270      5280               
AACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCA               
          ^   ^^     ^^  ^  ^  ^ ^^                ^^                      
          Mse I      Alw I     Mbo II              BspH I                  
              Dra I   Mbo I      BstY I             Nla III                
               Mse I     Mae I   Mfl I                                     
                     Xho II      Xho II                                    
                     HinGU II     SauIIIA I                                
                     BstY I                                                
                      Nde II      Nde II                                   
                      Dpn I       Mbo I                                    
                      SauIIIA I   Dpn I                                    
                            Hph I                                          
      5290      5300      5310      5320      5330      5340               
AAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAG               
       ^  ^                 ^                       ^      ^               
       Mse I                Dde I                   Mbo I                  
          Mea II                                    SauIIIA I              
                                                    Nde II                 
                                                    Dpn I                  
                                                           Alw I           
                                                           HinGU II        
                                                           BstY I          
                                                           Xho II          
      5350      5360      5370      5380      5390      5400               
GATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCAC               
^         ^^             ^^         ^                                      
Dpn I     Xho II         HinP I     Fnu4H I                                
SauIIIA I                Cfo I                                             
Mbo I     Mfl I          Hha I                                             
Nde II    BstY I          FnuD II                                          
           Nde II         Mvn I                                            
           Dpn I          Tha I                                            
           SauIIIA I      BstU I                                           
           Mbo I          Acc II                                           
      5410      5420      5430      5440      5450      5460               
CGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAA               
                       ^ ^^      ^                      ^  ^               
                       Hap II    Alu I                  Mae III            
                       Msp I                               Bsr I           
                       Hpa II                                              
                         Alw I                                             
                         HinGU II                                          
                          Nde II                                           
                          Mbo I                                            
                          SauIIIA I                                        
                          Dpn I                                            
      5470      5480      5490      5500      5510      5520               
CTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCC               
              ^                       ^                ^^                  
              Hha I                   Mae I            Hae I               
              HinP I                                    Hae III            
              Cfo I                                     Pal I              
      5530      5540      5550      5560      5570      5580               
ACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAG               
                                  ^                 ^    ^                 
                                  Mnl I             Mae III                
                                                         Alw NI            
      5590      5600      5610      5620      5630      5640               
TGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTAC               
  ^  ^                          ^^       ^             ^   ^               
  Fnu4H I                       Cfr 10I                Mae III             
     Fnu4H I                     Msp I   Hinf I            Hpa II          
                                 Hpa II                    Msp I           
                                 Hap II                    Hap II          
                                 Bcn I                                     
                                 Dsa V                                     
                                 ScrF I                                    
      5650      5660      5670      5680      5690      5700               
CGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGC               
        ^ ^                          ^            ^                        
        Cfo I                        Sno I        Alu I                    
        HinP I                       Nsp II                                
        Hha I                        Apa LI                                
          Bbv I                      Asp HI                                
          Fnu4H I                    Bsp 1286 I                            
                                     HgiA I                                
      5710      5720      5730      5740      5750      5760               
GAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTC               
                 ^                  ^          ^^                          
                 Dde I              Alu I      Hae II                      
                                                HinP I                     
                                                Hha I                      
                                                Cfo I                      
      5770      5780      5790      5800      5810      5820               
CCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCA               
                          ^      ^                     ^                   
                          Hap II                       HinP I              
                          Hpa II                       Nsp II              
                          Msp I                        Bsp 1286 I          
                                 Fnu4H I               Hha I               
                                                       Cfo I               
      5830      5840      5850      5860      5870      5880               
CGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACC               
      ^    ^            ^                                 ^                
      Alu I             Dsa V                             Mnl I            
           Dsa V        EcoR II                                            
           ScrF I       Apy I                                              
           EcoR II      BstN I                                             
           BstN I       ScrF I                                             
           Apy I        Mva I                                              
           Mva I        Nci I                                              
           Nci I                                                           
      5890      5900      5910      5920      5930      5940               
TCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACG               
   ^         ^                            ^                                
   Drd I     Taq I                        Nla IV                           
      5950      5960      5970      5980      5990      6000               
CCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCT               
       ^^^^          ^   ^ ^^         ^^           ^^                      
       Mvn I         Nla IV           Eae IV       Afl III                 
       BstU I            Nci I        Cfr I         Nla III                
       FnuD II           Dsa V        Hae I                                
       Tha I             BstN I        Pal I                               
       Acc II            Mva I         Hae III                             
        Fnu4H I          Apy I                                             
         Eae IV          ScrF I                                            
         Cfr I           EcoR II                                           
          Hae III          Eae IV                                          
          Pal I            Hae I                                           
                           Cfr I                                           
                            Pal I                                          
                            Hae III                                        
      6010      6020      6030      6040      6050      6060               
TTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATA               
                 ^                                  ^                      
                 Hinf I                             Alu I                  
      6070      6080      6090      6100      6110      6120               
CCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGC               
      ^  ^               ^ ^    ^                    ^   ^^                
      Fnu4H I            Cfo I                       Mbo II                
         Fnu4H I         HinP I                          Hae II            
         Bbv I           Hha I                            Hha I            
                           Fnu4H I                        Bsp 1286 I       
                           Bbv I                          Cfo I            
                                Ple I                     HinP I           
                                Hinf I                    Nsp II           
                                                          Ban II           
      6130      6140      6150      6160      6170      6180               
GCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCTTA               
                 ^     ^ ^^^    ^^   ^    ^^    ^^^                        
                 Mnl I   Mvn I   Hae III   Mse I                           
                       Nsp III   Pal I    Ase I   /Asn I                   
                         BstU I                 Bbv I                      
                         FnuD II     Hinf I     Fnu4H I                    
                         Acc II                  Pvu II                    
                         Tha I                    Alu I                    
                          Hha I                                            
                          HinP I                                           
                          Cfo I                                            
                           Acc II                                          
                           Tha I                                           
                           Mvn I                                           
                           FnuD II                                         
                           BstU I                                          
                                Eae I                                      
      6190      6200      6210                                             
TCGAAATTAATACGACTCACTATAGGGAGACCGG                                         
^    ^^      ^                ^                                            
Taq I        Hinf I           Msp I                                        
     Ase I   /Asn I           Hpa II                                       
      Mse I                   Hap II                                       

This page is produced and maintained by Ray and Peter
Last updated Monday, April 15, 1996 - 12:56:17