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On this page you will find some of the molecular biology protocols I used to use in the lab (most are out of date by now). They do work well though and are in some cases superior to current (mostly kit based) ones. Either way, I am keeping this page for mostly historical reasons.

        ELISA for FMuLV p30
        Total RNA isolation
        Lysis by boiling (fast minipreps)
        Mini prep alkaline lysis
        Plasmid mini prep for sequencing I
        Plasmid mini preps for sequencing II
        How to blunt DNA ends
        Cat assay
        Cat assay (liquid phase)
        Glass technique
        Maxi prep (CsCL)
        Phenol extraction
        Transformation of ligation

This page is produced and maintained by Peter
Last updated July 1, 2009